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Survival and differentiation of neuroepithelial stem cells following transplantation into the lateral ventricle of rats.
- Source :
-
The Chinese journal of physiology [Chin J Physiol] 2008 Aug 31; Vol. 51 (4), pp. 247-51. - Publication Year :
- 2008
-
Abstract
- Neuroepithelial stem cells (NEPs) demonstrate a high potential for self-renewal and differentiation during embryonic development. To explore the survival and differentiation of NEPs in vivo, we isolated NEPs from green fluorescence protein (GFP) transgenic embryos and transplanted into the lateral ventricle of rats. In vitro culture, NEPs proliferated into neurospheres and differentiated into both neurons and glia. When transplanted into the lateral ventricle of rats, these GFP positive NEPs (GFP+ NEPs) survived and attached to the wall of ventricle. Moreover, grafted cells differentiated into neuron-specific enolase (NSE) positive neurons and glial fibrillary acidic protein (GFAP) positive astrocytes and migrated into the host brain. Thus, our results indicate that NEPs can survive and differentiate into neurons and astrocytes in the lateral ventricle following transplantation.
- Subjects :
- Animals
Astrocytes cytology
Astrocytes metabolism
Cell Differentiation physiology
Cell Survival physiology
Cells, Cultured
Glial Fibrillary Acidic Protein metabolism
Green Fluorescent Proteins genetics
Immunohistochemistry
Neuroepithelial Cells metabolism
Phosphopyruvate Hydratase metabolism
Rats
Rats, Transgenic
Rats, Wistar
Lateral Ventricles cytology
Neuroepithelial Cells cytology
Stem Cell Transplantation
Stem Cells cytology
Subjects
Details
- Language :
- English
- ISSN :
- 0304-4920
- Volume :
- 51
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- The Chinese journal of physiology
- Publication Type :
- Academic Journal
- Accession number :
- 19112882