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[Effect of betulinic acid on proliferation and apoptosis in Jurkat cells and its mechanism].
[Effect of betulinic acid on proliferation and apoptosis in Jurkat cells and its mechanism].
- Source :
-
Zhonghua zhong liu za zhi [Chinese journal of oncology] [Zhonghua Zhong Liu Za Zhi] 2008 Aug; Vol. 30 (8), pp. 588-92. - Publication Year :
- 2008
-
Abstract
- Objective: To investigate the anticancer effects of betulinic acid (BA) on Jurkat cells in vitro and its molecular mechanism.<br />Methods: The effects of betulinic acid on the growth of Jurkat cells were studied by 3-(4, 5-dimethyl-2-thiazolyl)-2, 5diphenyl-2H-tetrazolium (MTT) assay. Apoptosis was assessed by Hoechst33258 staining and annexin-V/PI double-labeled cytometry. The effect of betulinic acid on the cell cycle of Jurkat cells was studied by propidium iodide staining. RT-PCR and Western blot were used to analyze the changes of cyclin D3, bcl-xl mRNA and protein levels in Jurkat cells after treatment with betulinic acid.<br />Results: The proliferation of Jurkat cells was decreased in betulinic acid-treated group at a 24 h IC50 value of 70.0 micromol/L. The effect of betulinic acid to induce apoptosis in Jurkat cells was in a time- and dose-dependent manner. Jurkat cells treated with betulinic acid showed an increase of G0/G1 phase and decrease of S phase. The Jurkat cells treated with 0, 20, 60, 100 micromol/L betulinic acid for 24 h, showed an increased G0/G1 phase from 31.0% to 58.8%, whereas decreased S phase from 61.5% to 35.8%, respectively. PBMC was less sensitive to the cytotoxic effect of betulinic acid than Jurkat cells. The expression of cyclin D3, bcl-xl mRNA and protein were decreased sharply in Jurkat cells treated with betulinic acid.<br />Conclusion: Betulinic acid can inhibit the proliferation of Jurkat cells by regulating the cell cycle that arrests cells at G0/G1 phase and induces apoptosis in Jurkat cells. The antitumor effects of betulinic acid may be related to down-regulation of the expression of cyclin D3 and bcl-xl.
- Subjects :
- Antineoplastic Agents, Phytogenic pharmacology
Betula chemistry
Cell Cycle drug effects
Cyclin D3 genetics
Gene Expression Regulation, Neoplastic
Humans
Jurkat Cells
Pentacyclic Triterpenes
Plant Bark chemistry
Plants, Medicinal chemistry
RNA, Messenger metabolism
bcl-X Protein genetics
Betulinic Acid
Apoptosis drug effects
Cell Proliferation drug effects
Cyclin D3 metabolism
Triterpenes pharmacology
bcl-X Protein metabolism
Subjects
Details
- Language :
- Chinese
- ISSN :
- 0253-3766
- Volume :
- 30
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Zhonghua zhong liu za zhi [Chinese journal of oncology]
- Publication Type :
- Academic Journal
- Accession number :
- 19102935