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[Effect of betulinic acid on proliferation and apoptosis in Jurkat cells and its mechanism].

[Effect of betulinic acid on proliferation and apoptosis in Jurkat cells and its mechanism].

Authors :
Chen Z
Wu QL
Chen Y
He J
Source :
Zhonghua zhong liu za zhi [Chinese journal of oncology] [Zhonghua Zhong Liu Za Zhi] 2008 Aug; Vol. 30 (8), pp. 588-92.
Publication Year :
2008

Abstract

Objective: To investigate the anticancer effects of betulinic acid (BA) on Jurkat cells in vitro and its molecular mechanism.<br />Methods: The effects of betulinic acid on the growth of Jurkat cells were studied by 3-(4, 5-dimethyl-2-thiazolyl)-2, 5diphenyl-2H-tetrazolium (MTT) assay. Apoptosis was assessed by Hoechst33258 staining and annexin-V/PI double-labeled cytometry. The effect of betulinic acid on the cell cycle of Jurkat cells was studied by propidium iodide staining. RT-PCR and Western blot were used to analyze the changes of cyclin D3, bcl-xl mRNA and protein levels in Jurkat cells after treatment with betulinic acid.<br />Results: The proliferation of Jurkat cells was decreased in betulinic acid-treated group at a 24 h IC50 value of 70.0 micromol/L. The effect of betulinic acid to induce apoptosis in Jurkat cells was in a time- and dose-dependent manner. Jurkat cells treated with betulinic acid showed an increase of G0/G1 phase and decrease of S phase. The Jurkat cells treated with 0, 20, 60, 100 micromol/L betulinic acid for 24 h, showed an increased G0/G1 phase from 31.0% to 58.8%, whereas decreased S phase from 61.5% to 35.8%, respectively. PBMC was less sensitive to the cytotoxic effect of betulinic acid than Jurkat cells. The expression of cyclin D3, bcl-xl mRNA and protein were decreased sharply in Jurkat cells treated with betulinic acid.<br />Conclusion: Betulinic acid can inhibit the proliferation of Jurkat cells by regulating the cell cycle that arrests cells at G0/G1 phase and induces apoptosis in Jurkat cells. The antitumor effects of betulinic acid may be related to down-regulation of the expression of cyclin D3 and bcl-xl.

Details

Language :
Chinese
ISSN :
0253-3766
Volume :
30
Issue :
8
Database :
MEDLINE
Journal :
Zhonghua zhong liu za zhi [Chinese journal of oncology]
Publication Type :
Academic Journal
Accession number :
19102935