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A haemagglutinin from the medicinal fungus Cordyceps militaris.
- Source :
-
Bioscience reports [Biosci Rep] 2009 Jun 25; Vol. 29 (5), pp. 321-7. Date of Electronic Publication: 2009 Jun 25. - Publication Year :
- 2009
-
Abstract
- There are only a few reports on agglutinins from ascomycete and medicinal fungi. An HA (haemagglutinin), with an N-terminal amino acid sequence different from those of known lectins, was isolated in the present study from dried fruiting bodies of the medicinal ascomycete fungus Cordyceps militaris. The purification protocol consisted of affinity chromatography, ion-exchange chromatography and gel filtration. The haemagglutinating activity of the HA could not be inhibited by simple sugars or heparin, and was stable over the pH range 2-13 and up to 60 degrees C. Chemical modification of tryptophan and tyrosine residues had no effect. The HA exhibited some antiproliferative activity towards hepatoma (HepG2) cells and inhibited HIV-1 reverse transcriptase (IC50=10 microM). However, it did not exhibit antifungal activity, mitogenic activity towards splenocytes, nitric oxide-inducing activity towards macrophages or RNase activity. The results of the present study add to the meagre information pertaining to agglutinins from ascomycete and medicinal mushrooms. It is revealed in this study that C. militaris HA differs from other ascomycete mushroom HAs in a variety of biochemical characteristics.
- Subjects :
- Carcinoma, Hepatocellular drug therapy
Cell Line, Tumor
Cell Proliferation drug effects
Dose-Response Relationship, Drug
Fruiting Bodies, Fungal chemistry
HIV Reverse Transcriptase metabolism
Hemagglutinins analysis
Hemagglutinins chemistry
Hemagglutinins pharmacology
Humans
Inhibitory Concentration 50
Liver Neoplasms drug therapy
Microbial Sensitivity Tests
Molecular Weight
Protein Stability
Ribonucleases analysis
Ribonucleases metabolism
Sequence Analysis, Protein
Temperature
Tryptophan chemistry
Tyrosine chemistry
Cordyceps chemistry
Hemagglutinins isolation & purification
Subjects
Details
- Language :
- English
- ISSN :
- 1573-4935
- Volume :
- 29
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Bioscience reports
- Publication Type :
- Academic Journal
- Accession number :
- 19093913
- Full Text :
- https://doi.org/10.1042/BSR20080153