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Histone H2B deubiquitination is required for transcriptional activation of FLOWERING LOCUS C and for proper control of flowering in Arabidopsis.
- Source :
-
Plant physiology [Plant Physiol] 2009 Feb; Vol. 149 (2), pp. 1196-204. Date of Electronic Publication: 2008 Dec 17. - Publication Year :
- 2009
-
Abstract
- The spectrum of histone modifications at a given locus is a critical determinant for the correct output of gene expression. In Arabidopsis (Arabidopsis thaliana), many studies have examined the relationship between histone methylation and gene expression, but few studies exist on the relationship between other covalent histone modifications and gene expression. In this work, we describe the role of histone H2B deubiquitination in the activation of gene expression and the consequence of a perturbation of histone H2B deubiquitination in the timing of the floral transition in Arabidopsis. A mutation in a H2B deubiquitinase, UBIQUITIN-SPECIFIC PROTEASE26 (UBP26), results in an early-flowering phenotype. In the ubp26 mutant, mRNA levels of the floral repressor FLOWERING LOCUS C (FLC) and other related family members is decreased. Furthermore, this mutant accumulates H2B monoubiquitination, and has decreased levels of H3K36 trimethylation and increased levels of H3K27 trimethylation at the FLC locus. Thus, UBP26 is required for transcriptional activation of FLC through H2B deubiquitination and is consistent with a model in which deubiquitination is necessary for the accumulation of H3K36 trimethylation and the proper level of transcriptional activation.
- Subjects :
- Arabidopsis enzymology
Arabidopsis metabolism
Endopeptidases genetics
Exons
Flowers genetics
Gene Expression Regulation, Plant
Genome, Plant
Phenotype
Saccharomyces cerevisiae genetics
Transcriptional Activation
Ubiquitin-Specific Proteases
Arabidopsis genetics
Arabidopsis Proteins genetics
Histones metabolism
MADS Domain Proteins genetics
Transcription, Genetic
Ubiquitin metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0032-0889
- Volume :
- 149
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Plant physiology
- Publication Type :
- Academic Journal
- Accession number :
- 19091875
- Full Text :
- https://doi.org/10.1104/pp.108.131508