Effect of growth temperature and pH on the aminopeptidase activity of Pseudomonas putida, P. fluorescens and Flavobacterium odoratum; the 4-nitroaniline test is reliable.

Authors :: Lopez-Tomas LA
Ordoñez JA
Mediavilla C
Rodriguez-Marin JL
Sarmiento P
Zamora A
Garcia de Fernando G
Source :: Folia microbiologica [Folia Microbiol (Praha)] 2008; Vol. 53 (5), pp. 423-6. Date of Electronic Publication: 2008 Dec 16.
Publication Year :: 2008
Abstract: No significant difference (p > 0.05) was observed in the specific aminopeptidase activity (SAA) developed by Pseudomonas fluorescens, P. putida and Flavobacterium odoratum either growing at pH 5.0-6.5 or at 7 and 12 degrees C. Nevertheless, a significant difference was found when comparing the SAA of these organisms. The SAA of F. odoratum was lower than those of pseudomonads. The 4-nitroaniline test is reliable to estimate the G(-) load of fresh food products.

Subjects :: Bacterial Proteins metabolism
Bacteriological Techniques
Culture Media
Flavobacterium growth & development
Hydrogen-Ion Concentration
Pseudomonas fluorescens growth & development
Pseudomonas putida growth & development
Temperature
Aminopeptidases metabolism
Aniline Compounds metabolism
Flavobacterium enzymology
Pseudomonas fluorescens enzymology
Pseudomonas putida enzymology

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