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Preparation and evaluation of propranolol molecularly imprinted solid-phase microextraction fiber for trace analysis of beta-blockers in urine and plasma samples.
- Source :
-
Journal of chromatography. A [J Chromatogr A] 2009 Jan 09; Vol. 1216 (2), pp. 190-7. Date of Electronic Publication: 2008 Nov 30. - Publication Year :
- 2009
-
Abstract
- An improved multiple co-polymerization technique was developed to prepare a novel molecularly imprinted polymer (MIP)-coated solid-phase microextraction (SPME) fiber with propranolol as template. Investigation was performed for the characteristics and application of the fibers. The MIP coating was highly crosslinked and porous with the average thickness of only 25.0 microm. Consequently, the adsorption and desorption of beta-blockers within the MIP coating could be achieved quickly. The specific selectivity was discovered with the MIP-coated fibers to propranolol and its structural analogues such as atenolol, pindolol, and alprenolol. In contrast, only non-specific adsorption could be shown with the non-imprinted polymer (NIP)-coated fibers, and the extraction efficiencies of propranolol and pindolol with the MIP-coated fibers were higher markedly than that with the commercial SPME fibers. A MIP-coated SPME coupled with high-performance liquid chromatography (HPLC) method for propranolol and pindolol determination was developed under the optimized extraction conditions. Linear ranges for propranolol and pindolol were 20-1000 microg L(-1) and detection limits were 3.8 and 6.9 microg L(-1), respectively. Propranolol and pindolol in the spiked human urine and plasma samples, extracted with organic solvent firstly, could be simultaneous monitored with satisfactory recoveries through this method.
- Subjects :
- Adsorption
Alprenolol analysis
Alprenolol chemistry
Atenolol analysis
Atenolol chemistry
Chromatography, High Pressure Liquid
Humans
Pindolol blood
Pindolol chemistry
Pindolol urine
Polymers chemical synthesis
Polymers chemistry
Propranolol blood
Propranolol chemistry
Propranolol urine
Reproducibility of Results
Sensitivity and Specificity
Adrenergic beta-Antagonists blood
Adrenergic beta-Antagonists urine
Molecular Imprinting methods
Pindolol analysis
Propranolol analysis
Solid Phase Microextraction methods
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9673
- Volume :
- 1216
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of chromatography. A
- Publication Type :
- Academic Journal
- Accession number :
- 19084232
- Full Text :
- https://doi.org/10.1016/j.chroma.2008.11.064