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Ghrelin, cholecystokinin, and peptide YY in Atlantic salmon (Salmo salar): molecular cloning and tissue expression.
- Source :
-
General and comparative endocrinology [Gen Comp Endocrinol] 2009 Feb 01; Vol. 160 (3), pp. 223-35. Date of Electronic Publication: 2008 Dec 06. - Publication Year :
- 2009
-
Abstract
- Gastrointestinal (GI) peptide hormones, ghrelin (GHRL), cholecystokinin (CCK), and peptide YY (PYY) genes were identified in Atlantic salmon, Salmo salar. Full-length cDNAs encoding two isoforms of GHRL (GHRL-1 and GHRL-2), two isoforms of CCK (CCK-L and CCK-N) and peptide YY (PYY) cDNA were obtained. The GHRL-1 and GHRL-2 genes encoded proteins of 111- and 108-amino acids, respectively. Both types of GHRL were mainly expressed in the stomach, but also weakly expressed in the pyloric caeca, mid-gut, adipose tissue, and testis. The CCK-L and CCK-N genes encoded preproproteins of 132- and 140-amino acids, respectively. Both types of CCK were strongly expressed in the brain and comparatively weakly expressed in other tissues, including the digestive tract. In the digestive tract, CCK-L was mainly expressed in the pyloric caeca and hind-gut, while CCK-N was only expressed in the pyloric caeca. The PYY gene encoded for 97-amino acid residues and was mainly expressed in the brain and anterior part of the intestine, including the pyloric caeca. In an experiment, we demonstrated that 6 days starvation led to, increased GHRL-1 mRNA levels in the GI tract (stomach), while there no significant changes in expression levels for the other hormones in the GI tract. This suggests an orexigenic role for GHRL-1 in Atlantic salmon. These data contribute to elucidate the functional relationships among teleost gastrointestinal peptide hormones.
- Subjects :
- Amino Acid Sequence
Animals
Base Sequence
Cholecystokinin chemistry
Cholecystokinin genetics
Cloning, Molecular
DNA, Complementary chemistry
Fish Proteins chemistry
Fish Proteins genetics
Ghrelin chemistry
Ghrelin genetics
Molecular Sequence Data
Peptide YY chemistry
Peptide YY genetics
Phylogeny
Salmo salar genetics
Sequence Alignment
Sequence Analysis, DNA
Sequence Analysis, Protein
Starvation metabolism
Cholecystokinin metabolism
Fish Proteins metabolism
Ghrelin metabolism
Peptide YY metabolism
Salmo salar metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1095-6840
- Volume :
- 160
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- General and comparative endocrinology
- Publication Type :
- Academic Journal
- Accession number :
- 19073185
- Full Text :
- https://doi.org/10.1016/j.ygcen.2008.11.024