[Effect of Atrazine on the content of calcium and expression of calmodulin in splenocytes of mice].

Objective: To investigate the effects of Atrazine on the concentration of intracellular calcium ([Ca2+]i) and the expression of calmodulin (CaM) in splenocytes.
Methods: The mice were divided into 5 groups, and were lavaged the doses of 100, 200 and 400 mg/kg Atrazine respectively. The mice were administrated Atrazine for 3 weeks. The Ca2+ concentrations in serum were detected by atomic absorption spectrophotometer. The [Ca2+]i in splenocytes were determined by fluorospectrophotometer. The expressions of CaM were detected by western blot analysis.
Results: The changes of the Ca2+ concentrations of serum in treatment groups were not observed. The intracellular calcium concentrations and CaM expressions in treatment groups were significantly increased and decreased compared with the control group.
Conclusion: Atrazine could induced the increase of calcium concentration and the depression of the expression of CaM in splenocytes of mice.