Back to Search
Start Over
The Kv1.2 potassium channel: the position of an N-glycan on the extracellular linkers affects its protein expression and function.
- Source :
-
Brain research [Brain Res] 2009 Jan 28; Vol. 1251, pp. 16-29. Date of Electronic Publication: 2008 Nov 20. - Publication Year :
- 2009
-
Abstract
- Voltage-gated potassium Kv1 channels have three extracellular linkers, the S1-S2, the S3-S4, and the S5-P. The S1-S2 is the only linker that has an N-glycan and it is at a conserved position on this linker on Kv1.1-Kv1.5 and Kv1.7 channels. We hypothesize that an N-glycan is found at only this position due to its effect on folding, trafficking, and/or function of these channels. To investigate this hypothesis, N-glycosylation sites were engineered at different positions on the extracellular linkers of Kv1.2 to determine the effects of N-glycans on channel surface protein expression and function. Our data suggest that for Kv1 channels, (1) placing an N-glycan at non-native positions on the S1-S2 linker decreased cell surface protein expression but the N-glycan still affected function similarly as if it were at its native position, (2) placing a non-native N-glycan on the S3-S4 linker significantly altered function, and (3) placing a non-native N-glycan on the S5-P linker disrupted both trafficking and function. We suggest that Kv1 channels have an N-glycan at a conserved position on only the S1-S2 linker to overcome the constraints for proper folding, trafficking, and function that appear to occur if the N-glycan is moved from this position.
- Subjects :
- Amino Acid Sequence physiology
Animals
CHO Cells
Cell Membrane genetics
Cricetinae
Cricetulus
Extracellular Space metabolism
Glycosaminoglycans metabolism
Glycosylation
Kv1.2 Potassium Channel chemistry
Kv1.2 Potassium Channel genetics
Membrane Potentials physiology
Potassium metabolism
Protein Stability
Protein Structure, Tertiary physiology
Protein Transport physiology
Rats
Cell Membrane chemistry
Cell Membrane metabolism
Kv1.2 Potassium Channel metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1872-6240
- Volume :
- 1251
- Database :
- MEDLINE
- Journal :
- Brain research
- Publication Type :
- Academic Journal
- Accession number :
- 19056359
- Full Text :
- https://doi.org/10.1016/j.brainres.2008.11.033