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Isolation and characterization of a gene coding for chitin deacetylase specifically expressed during fruiting body development in the basidiomycete Flammulina velutipes and its expression in the yeast Pichia pastoris.
- Source :
-
FEMS microbiology letters [FEMS Microbiol Lett] 2008 Dec; Vol. 289 (2), pp. 130-7. - Publication Year :
- 2008
-
Abstract
- Fv-pda, a gene coding for chitin deacetylase (CDA), was isolated from the basidiomycete Flammulina velutipes by differential display targeted for genes specifically expressed during fruiting body development. The fv-pda ORF comprises 250 amino acid residues and is interrupted by 10 introns. The fv-pda cDNA was expressed in the yeast Pichia pastoris, and the resulting recombinant FV-PDA was used for enzymatic characterization. The recombinant FV-PDA catalyses deacetylation of N-acetyl-chitooligomers, from dimer to pentamer, glycol chitin and colloidal chitin. The fv-pda was specifically expressed through the entire stage of fruiting body development, and the transcript was abundant in stipes of mature fruiting bodies. These results suggest that CDA plays an important role in the process of fruiting of F. velutipes.
- Subjects :
- Amidohydrolases genetics
Amidohydrolases metabolism
Amino Acid Sequence
Base Sequence
Flammulina chemistry
Flammulina enzymology
Flammulina genetics
Fruiting Bodies, Fungal chemistry
Fruiting Bodies, Fungal genetics
Fruiting Bodies, Fungal growth & development
Fungal Proteins genetics
Fungal Proteins metabolism
Molecular Sequence Data
Pichia metabolism
Sequence Alignment
Substrate Specificity
Amidohydrolases chemistry
Flammulina growth & development
Fruiting Bodies, Fungal enzymology
Fungal Proteins chemistry
Gene Expression
Gene Expression Regulation, Enzymologic
Pichia genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0378-1097
- Volume :
- 289
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- FEMS microbiology letters
- Publication Type :
- Academic Journal
- Accession number :
- 19054103
- Full Text :
- https://doi.org/10.1111/j.1574-6968.2008.01361.x