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Effect of progesterone on proinflammatory cytokine production by monocytes stimulated with pathogens associated with preterm birth.
- Source :
-
American journal of reproductive immunology (New York, N.Y. : 1989) [Am J Reprod Immunol] 2008 Oct; Vol. 60 (4), pp. 346-53. - Publication Year :
- 2008
-
Abstract
- Problem: A number of clinical trials have demonstrated that supplemental progesterone (P4) can prevent preterm birth. Although P4 can decrease the production of mediators of inflammation by lipopolysaccharide (LPS)-stimulated macrophages, a majority of infections associated with preterm birth are due to Ureaplasma urealyticum, which does not contain LPS. Therefore, we studied whether P4 could lower the production of proinflammatory cytokines by monocytes stimulated with U. urealyticum.<br />Method of Study: Human monocytes (THP-1 cells) were treated with P4 and then stimulated with heat-killed Escherichia coli or U. urealyticum. Cytokine concentrations in the conditioned medium were then measured by ELISA and relative viability of the cells was assessed colorimetrically. The impact of P4 on interleukin (IL)-1beta, tumor necrosis factor-alpha (TNF-alpha) and IL-8 production was assessed by comparing levels across different P4 dosages and organism concentrations.<br />Results: Both organisms increased IL-1alpha, TNF-alpha and IL-8 production in a dose-dependent manner. P4 enhanced the production of IL-1beta and IL-8, but inhibited TNF-alpha production by monocytes stimulated with either organism.<br />Conclusion: P4 modulates cytokine production by E. coli and U. urealyticum-stimulated monocytes in a similar manner and is not strictly immunosuppressive. This suggests that P4 does not prevent preterm birth by simply suppressing bacteria-stimulated cytokine production.
- Subjects :
- Cytokines biosynthesis
Escherichia coli pathogenicity
Female
Humans
Interleukin-1beta biosynthesis
Interleukin-1beta drug effects
Interleukin-1beta immunology
Interleukin-8 biosynthesis
Interleukin-8 drug effects
Interleukin-8 immunology
Monocytes immunology
Pregnancy
Premature Birth microbiology
Tumor Necrosis Factor-alpha biosynthesis
Tumor Necrosis Factor-alpha immunology
Ureaplasma urealyticum pathogenicity
Cytokines immunology
Monocytes drug effects
Premature Birth immunology
Progesterone pharmacology
Progestins pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1046-7408
- Volume :
- 60
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- American journal of reproductive immunology (New York, N.Y. : 1989)
- Publication Type :
- Academic Journal
- Accession number :
- 19046141
- Full Text :
- https://doi.org/10.1111/j.1600-0897.2008.00633.x