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Antiviral activity of lauryl gallate against animal viruses.
- Source :
-
Antiviral therapy [Antivir Ther] 2008; Vol. 13 (7), pp. 909-17. - Publication Year :
- 2008
-
Abstract
- Background: Antiviral compounds are needed in the control of many animal and human diseases.<br />Methods: We analysed the effect of the antitumoural drug lauryl gallate on the infectivity of the African swine fever virus among other DNA (herpes simplex and vaccinia) and RNA (influenza, porcine transmissible gastroenteritis and Sindbis) viruses, paying attention to its effect on the viability of the corresponding host cells.<br />Results: Viral production was strongly inhibited in different cell lines at non-toxic concentrations of the drug (1-10 microM), reducing the titres 3->5 log units depending on the multiplicity of infection. In our model system (African swine fever virus in Vero cells), the addition of the drug 1 h before virus adsorption completely abolished virus productivity in a one-step growth virus cycle. Interestingly, no inhibitory effect was observed when lauryl gallate was added after 5-8 h post-infection. Both cellular and viral DNA synthesis and late viral transcription were inhibited by the drug; however, the early viral protein synthesis and the virus-mediated increase of p53 remained unaffected. Activation of the apoptotic effector caspase-3 was not detected after lauryl gallate treatment of Vero cells. Furthermore, the presence of the drug abrogated the activation of this protease induced by the virus infection.<br />Conclusions: Lauryl gallate is a powerful antiviral agent against several pathogens of clinical and veterinary importance. The overall results indicate that a cellular factor or function might be the target of the antiviral action of alkyl gallates.
- Subjects :
- African Swine Fever Virus drug effects
African Swine Fever Virus physiology
Animals
Cell Line
Chlorocebus aethiops
Cricetinae
DNA Viruses physiology
DNA, Viral biosynthesis
DNA, Viral drug effects
Gallic Acid pharmacology
Proteins drug effects
Proteins genetics
Proteins metabolism
RNA Viruses drug effects
RNA Viruses physiology
Vero Cells
Viral Proteins drug effects
Viral Proteins genetics
Viral Proteins metabolism
Virus Replication
Antiviral Agents pharmacology
DNA Viruses drug effects
Gallic Acid analogs & derivatives
Subjects
Details
- Language :
- English
- ISSN :
- 1359-6535
- Volume :
- 13
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Antiviral therapy
- Publication Type :
- Academic Journal
- Accession number :
- 19043925