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In vivo alternative testing with zebrafish in ecotoxicology.

Authors :
Seok SH
Baek MW
Lee HY
Kim DJ
Na YR
Noh KJ
Park SH
Lee HK
Lee BH
Park JH
Source :
Journal of veterinary science [J Vet Sci] 2008 Dec; Vol. 9 (4), pp. 351-7.
Publication Year :
2008

Abstract

Although rodents have previously been used in ecotoxicological studies, they are expensive, time-consuming, and are limited by strict legal restrictions. The present study used a zebrafish (Danio rerio) model and generated data that was useful for extrapolating toxicant effects in this system to that of humans. Here we treated embryos of the naive-type as well as a transiently transfected zebrafish liver cell line carrying a plasmid (phAhREEGFP), for comparing toxicity levels with the well-known aryl hydrocarbon receptor (AhR)-binding toxicants: 3,3',4,4',5-pentachlorobiphenyl (PCB126), 2,3,7,8-tetrachlorodibenzo-p-dioxin, and 3-methylcholanthrene. These toxicants induced a concentration-dependent increase in morphological disruption, indicating toxicity at early life-stages. The transient transgenic zebrafish liver cell line was sensitive enough to these toxicants to express the CYP1A1 regulated enhanced green fluorescent protein. The findings of this study demonstrated that the zebrafish in vivo model might allow for extremely rapid and reproducible toxicological profiling of early life-stage embryo development. We have also shown that the transient transgenic zebrafish liver cell line can be used for research on AhR mechanism studies.

Details

Language :
English
ISSN :
1229-845X
Volume :
9
Issue :
4
Database :
MEDLINE
Journal :
Journal of veterinary science
Publication Type :
Academic Journal
Accession number :
19043309
Full Text :
https://doi.org/10.4142/jvs.2008.9.4.351