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In vivo alternative testing with zebrafish in ecotoxicology.
- Source :
-
Journal of veterinary science [J Vet Sci] 2008 Dec; Vol. 9 (4), pp. 351-7. - Publication Year :
- 2008
-
Abstract
- Although rodents have previously been used in ecotoxicological studies, they are expensive, time-consuming, and are limited by strict legal restrictions. The present study used a zebrafish (Danio rerio) model and generated data that was useful for extrapolating toxicant effects in this system to that of humans. Here we treated embryos of the naive-type as well as a transiently transfected zebrafish liver cell line carrying a plasmid (phAhREEGFP), for comparing toxicity levels with the well-known aryl hydrocarbon receptor (AhR)-binding toxicants: 3,3',4,4',5-pentachlorobiphenyl (PCB126), 2,3,7,8-tetrachlorodibenzo-p-dioxin, and 3-methylcholanthrene. These toxicants induced a concentration-dependent increase in morphological disruption, indicating toxicity at early life-stages. The transient transgenic zebrafish liver cell line was sensitive enough to these toxicants to express the CYP1A1 regulated enhanced green fluorescent protein. The findings of this study demonstrated that the zebrafish in vivo model might allow for extremely rapid and reproducible toxicological profiling of early life-stage embryo development. We have also shown that the transient transgenic zebrafish liver cell line can be used for research on AhR mechanism studies.
- Subjects :
- Animals
Benz(a)Anthracenes toxicity
Cell Line
Green Fluorescent Proteins
Hepatocytes cytology
Hepatocytes physiology
Larva drug effects
Larva growth & development
Lethal Dose 50
Methylcholanthrene
Polychlorinated Biphenyls toxicity
Polychlorinated Dibenzodioxins toxicity
Water Pollutants, Chemical adverse effects
Zebrafish physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1229-845X
- Volume :
- 9
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Journal of veterinary science
- Publication Type :
- Academic Journal
- Accession number :
- 19043309
- Full Text :
- https://doi.org/10.4142/jvs.2008.9.4.351