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NaCl induced high cationic hydroxyethylated cholesterol-based nanoparticle-mediated synthetic small interfering RNA transfer into prostate carcinoma PC-3 cells.
NaCl induced high cationic hydroxyethylated cholesterol-based nanoparticle-mediated synthetic small interfering RNA transfer into prostate carcinoma PC-3 cells.
- Source :
-
Biological & pharmaceutical bulletin [Biol Pharm Bull] 2008 Dec; Vol. 31 (12), pp. 2294-301. - Publication Year :
- 2008
-
Abstract
- The RNA interference (RNAi) effect is an alternative technology to antisense DNA as an experimental method of down-regulating a specific target protein. Optimal gene therapy for tumors must deliver synthetic small interfering RNA (siRNA) to tumor cells with high efficiency and minimal toxicity. Previously, we reported that cationic nanoparticles composed of cholesteryl-3beta-carboxyamidoethylene-N-hydroxyethylamine and Tween 80 (NP-OH) could deliver plasmid DNA with high transfection efficiency. In this study, to apply NP-OH for siRNA transfection, we optimized the charge ratio (+/-) of NP-OH/siRNA and nanoplex-forming solution, and evaluated the transfection efficiency into PC-3 cells. Positively charged nanoplex prepared in the presence of sodium chloride exhibited efficient siRNA transfer. The distribution of siRNA after transfection was strongly detected both in the cytoplasm and nucleus. Furthermore, we demonstrated that NP-OH nanoplex of bcl-2 siRNA significantly inhibited tumor growth compared with control siRNA, and the efficacy was comparable to commercial products. The results of the experiments showed that NP-OH nanoparticles have potential as a viable vector candidate for synthetic siRNA delivery.
- Subjects :
- Blotting, Western
Cell Line, Tumor
Cell Proliferation drug effects
Cholesterol administration & dosage
Cholesterol chemistry
DNA, Neoplasm genetics
Electrochemistry
Electrophoresis, Polyacrylamide Gel
Flow Cytometry
Genes, bcl-2 genetics
Genetic Vectors
Humans
Luciferases genetics
Microscopy, Confocal
Nanoparticles
Plasmids genetics
Polysorbates
RNA, Small Interfering chemistry
Reverse Transcriptase Polymerase Chain Reaction
Surface-Active Agents
Transfection
Antineoplastic Agents administration & dosage
Antineoplastic Agents chemistry
Cholesterol analogs & derivatives
RNA, Small Interfering administration & dosage
RNA, Small Interfering pharmacology
Sodium Chloride pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0918-6158
- Volume :
- 31
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Biological & pharmaceutical bulletin
- Publication Type :
- Academic Journal
- Accession number :
- 19043216
- Full Text :
- https://doi.org/10.1248/bpb.31.2294