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Cell-surface modification of non-GMO without chemical treatment by novel GMO-coupled and -separated cocultivation method.
- Source :
-
Applied microbiology and biotechnology [Appl Microbiol Biotechnol] 2009 Feb; Vol. 82 (2), pp. 293-301. Date of Electronic Publication: 2008 Nov 28. - Publication Year :
- 2009
-
Abstract
- We developed a novel method to coat living non-genetically modified (GM) cells with functional recombinant proteins. First, we prepared GM yeast to secrete constructed proteins that have two domains: a functional domain and a binding domain that recognizes other cells. Second, we cocultivated GM and non-GM yeasts that share and coutilize the medium containing recombinant proteins produced by GM yeasts using a filter-membrane-separated cultivation reactor. We confirmed that GM yeast secreted enhanced green fluorescent protein (EGFP) fusion proteins to culture medium. After cocultivation, EGFP fusion proteins produced by GM yeast were targeted to non-GM yeast (Saccharomyces cerevisiae BY4741DeltaCYC8 strain) cell surface. Yeast cell-surface engineering is a useful method that enables the coating of GM yeast cell surface with recombinant proteins to produce highly stable and accumulated protein particles. The results of this study suggest that development of cell-surface engineering from GM organisms (GMOs) to living non-GMOs by our novel cocultivation method is possible.
- Subjects :
- Coculture Techniques
Green Fluorescent Proteins genetics
Green Fluorescent Proteins isolation & purification
Green Fluorescent Proteins metabolism
Membrane Proteins genetics
Microbial Viability
Organisms, Genetically Modified genetics
Organisms, Genetically Modified metabolism
Protein Transport
Recombinant Fusion Proteins genetics
Recombinant Fusion Proteins isolation & purification
Recombinant Fusion Proteins metabolism
Saccharomyces cerevisiae metabolism
Candida albicans metabolism
Culture Techniques methods
Genetic Engineering methods
Membrane Proteins metabolism
Saccharomyces cerevisiae genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1432-0614
- Volume :
- 82
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Applied microbiology and biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 19039583
- Full Text :
- https://doi.org/10.1007/s00253-008-1787-5