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p53-Repressed miRNAs are involved with E2F in a feed-forward loop promoting proliferation.
- Source :
-
Molecular systems biology [Mol Syst Biol] 2008; Vol. 4, pp. 229. Date of Electronic Publication: 2008 Nov 25. - Publication Year :
- 2008
-
Abstract
- Normal cell growth is governed by a complicated biological system, featuring multiple levels of control, often deregulated in cancers. The role of microRNAs (miRNAs) in the control of gene expression is now increasingly appreciated, yet their involvement in controlling cell proliferation is still not well understood. Here we investigated the mammalian cell proliferation control network consisting of transcriptional regulators, E2F and p53, their targets and a family of 15 miRNAs. Indicative of their significance, expression of these miRNAs is downregulated in senescent cells and in breast cancers harboring wild-type p53. These miRNAs are repressed by p53 in an E2F1-mediated manner. Furthermore, we show that these miRNAs silence antiproliferative genes, which themselves are E2F1 targets. Thus, miRNAs and transcriptional regulators appear to cooperate in the framework of a multi-gene transcriptional and post-transcriptional feed-forward loop. Finally, we show that, similarly to p53 inactivation, overexpression of representative miRNAs promotes proliferation and delays senescence, manifesting the detrimental phenotypic consequence of perturbations in this circuit. Taken together, these findings position miRNAs as novel key players in the mammalian cellular proliferation network.
- Subjects :
- Animals
Breast Neoplasms
Cellular Senescence
E2F1 Transcription Factor
Female
Gene Expression Regulation, Neoplastic
Humans
Male
Systems Biology
Cell Proliferation
E2F Transcription Factors physiology
Gene Regulatory Networks physiology
MicroRNAs physiology
Tumor Suppressor Protein p53 physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1744-4292
- Volume :
- 4
- Database :
- MEDLINE
- Journal :
- Molecular systems biology
- Publication Type :
- Academic Journal
- Accession number :
- 19034270
- Full Text :
- https://doi.org/10.1038/msb.2008.65