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Fine-mapping chromosomal loss at 9p21: correlation with prognosis in primary cutaneous diffuse large B-cell lymphoma, leg type.
- Source :
-
The Journal of investigative dermatology [J Invest Dermatol] 2009 May; Vol. 129 (5), pp. 1149-55. Date of Electronic Publication: 2008 Nov 20. - Publication Year :
- 2009
-
Abstract
- Primary cutaneous diffuse large B-cell lymphoma, leg type (PCLBCL, LT) is the most aggressive type of primary cutaneous B-cell lymphoma. In a recent study on 12 patients it was found that inactivation of CDKN2A by either deletion of 9p21.3 or promoter hypermethylation is correlated with a worse prognosis. In the present EORTC multicenter study, skin biopsies of 64 PCLBCL, LT patients were analyzed by multiplex ligation-dependent probe amplification to validate these previous results and to fine-map the losses in this region. Although no minimal common region of loss could be identified, most homozygous loss was observed in the CDKN2A gene (43 of 64; 67%) encoding p16 and p14ARF. Promoter hypermethylation of p16 and p14ARF was found in six and zero cases, respectively. Survival was markedly different between patients with versus without aberrations in the CDKN2A gene (5-year disease-specific survival 43 versus 70%; P=0.06). In conclusion, our results confirm that deletion of chromosome 9p21.3 is found in a considerable proportion of PCLBCL, LT patients and that inactivation of the CDKN2A gene is associated with an unfavorable prognosis. In most patients the deletion involves a large area of at least several kilobase pairs instead of a small minimal common region.
- Subjects :
- Aged
Aged, 80 and over
DNA Methylation genetics
Female
Gene Deletion
Humans
Leg
Male
Middle Aged
Prognosis
Reproducibility of Results
Chromosome Mapping
Chromosomes, Human, Pair 9 genetics
Cyclin-Dependent Kinase Inhibitor p16 genetics
Lymphoma, Large B-Cell, Diffuse diagnosis
Lymphoma, Large B-Cell, Diffuse genetics
Skin Neoplasms diagnosis
Skin Neoplasms genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1523-1747
- Volume :
- 129
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- The Journal of investigative dermatology
- Publication Type :
- Academic Journal
- Accession number :
- 19020554
- Full Text :
- https://doi.org/10.1038/jid.2008.357