Back to Search
Start Over
TWIST1 overexpression is associated with nodal invasion and male sex in primary colorectal cancer.
- Source :
-
Annals of surgical oncology [Ann Surg Oncol] 2009 Jan; Vol. 16 (1), pp. 78-87. Date of Electronic Publication: 2008 Nov 11. - Publication Year :
- 2009
-
Abstract
- Background: TWIST1 is a basic helix-loop-helix (bHLH) transcription factor that has been involved in tumor progression and metastasis in several cancer types, although no evidence has been provided yet on its implication in colorectal carcinogenesis.<br />Methods: We examined the expression pattern of TWIST1 messenger RNA (mRNA) in 54 colorectal cancer biopsies compared with each respective adjacent normal mucosa by real-time reverse-transcriptase polymerase chain reaction (RT-PCR) methodology.<br />Results: TWIST1 mRNA was found significantly overexpressed in colorectal cancer samples compared to nontumorous colon mucosa (P < 0.0001). Receiver operating characteristic (ROC) curve analysis demonstrated that TWIST1 mRNA levels are significantly increased in patients with nodal invasion and, interestingly, a significant correlation with patient sex was also found.<br />Conclusions: Evidence for upregulation of TWIST1 mRNA in colorectal cancer is provided, suggesting its implication in the onset of malignant progression of this disease. In addition, significant higher levels of TWIST1 mRNA were found in men than in women, suggesting a possible transcriptional regulation of TWIST1 by sexual hormones. The use of TWIST1 as a new prognostic marker of advanced malignancy, and as a potential therapeutic target in colorectal cancer, is proposed.
- Subjects :
- Aged
Blotting, Western
Female
Gene Expression Regulation, Neoplastic
Humans
Immunoenzyme Techniques
Lymph Nodes
Lymphatic Metastasis
Male
Neoplasm Invasiveness
Neoplasm Staging
Prognosis
Reverse Transcriptase Polymerase Chain Reaction
Sex Factors
Colorectal Neoplasms genetics
Colorectal Neoplasms pathology
Nuclear Proteins genetics
RNA, Messenger metabolism
Twist-Related Protein 1 genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1534-4681
- Volume :
- 16
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Annals of surgical oncology
- Publication Type :
- Academic Journal
- Accession number :
- 19002529
- Full Text :
- https://doi.org/10.1245/s10434-008-0166-x