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Assessment in vitro of the active hemostatic properties of wound dressings.
- Source :
-
Journal of biomedical materials research. Part B, Applied biomaterials [J Biomed Mater Res B Appl Biomater] 2009 May; Vol. 89 (2), pp. 536-542. - Publication Year :
- 2009
-
Abstract
- The development of actively hemostatic wound dressings for use in severe trauma remains a major public-health and military goal. But, although some manufacturers claim that existing dressings activate platelets and/or blood coagulation, mechanistic evidence is often lacking. We describe a method for assessing the active hemostatic properties of dressings in vitro, entailing measurement of the flow of recalcified platelet-rich plasma (PRP) through a dressing sample. If the dressing is hemostatically active, flow is reduced. This flow is then compared with the flow-through of PRP in which both platelet and coagulation function are blocked with EDTA. The ratio of the two generates a hemostatic index that ranges from 1.0 (no active hemostasis) to 0 (highly potent). The method is applicable to porous or semiporous dressings, whether fabric, sponge, fleece, or granules. For an active dressing, the test is easily modified to differentiate between the contributions of platelet and coagulation to overall hemostasis. The method is illustrated for fabrics, over-the-counter gauze and sponge dressings, collagen-based sheets, and an absorbent granule dressing. One active collagen dressing is used to illustrate discrimination between platelet and coagulation function. The ability to assess hemostatic properties may significantly enhance the development of advanced active dressings.<br /> ((c) 2008 Wiley Periodicals, Inc.)
- Subjects :
- Anticoagulants pharmacology
Biological Assay methods
Blood Platelets drug effects
Blood Platelets metabolism
Edetic Acid pharmacology
Hemostasis drug effects
Hemostasis physiology
Hemostatic Techniques
Humans
Materials Testing
Platelet-Rich Plasma metabolism
Bandages
Blood Coagulation drug effects
Hemostatics pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1552-4981
- Volume :
- 89
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of biomedical materials research. Part B, Applied biomaterials
- Publication Type :
- Academic Journal
- Accession number :
- 18985792
- Full Text :
- https://doi.org/10.1002/jbm.b.31245