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The Arabidopsis GRAS protein SCL14 interacts with class II TGA transcription factors and is essential for the activation of stress-inducible promoters.
- Source :
-
The Plant cell [Plant Cell] 2008 Nov; Vol. 20 (11), pp. 3122-35. Date of Electronic Publication: 2008 Nov 04. - Publication Year :
- 2008
-
Abstract
- The plant signaling molecule salicylic acid (SA) and/or xenobiotic chemicals like the auxin mimic 2,4-D induce transcriptional activation of defense- and stress-related genes that contain activation sequence-1 (as-1)-like cis-elements in their promoters. as-1-like sequences are recognized by basic/leucine zipper transcription factors of the TGA family. Expression of genes related to the SA-dependent defense program systemic acquired resistance requires the TGA-interacting protein NPR1. However, a number of as-1-containing promoters can be activated independently from NPR1. Here, we report the identification of Arabidopsis thaliana SCARECROW-like 14 (SCL14), a member of the GRAS family of regulatory proteins, as a TGA-interacting protein that is required for the activation of TGA-dependent but NPR1-independent SA- and 2,4-D-inducible promoters. Chromatin immunoprecipitation experiments revealed that class II TGA factors TGA2, TGA5, and/or TGA6 are needed to recruit SCL14 to promoters of selected SCL14 target genes identified by whole-genome transcript profiling experiments. The coding regions and the expression profiles of the SCL14-dependent genes imply that they might be involved in the detoxification of xenobiotics and possibly endogenous harmful metabolites. Consistently, plants ectopically expressing SCL14 showed increased tolerance to toxic doses of the chemicals isonicotinic acid and 2,4,6-triiodobenzoic acid, whereas the scl14 and the tga2 tga5 tga6 mutants were more susceptible. Hence, the TGA/SCL14 complex seems to be involved in the activation of a general broad-spectrum detoxification network upon challenge of plants with xenobiotics.
- Subjects :
- Arabidopsis drug effects
Arabidopsis metabolism
Arabidopsis Proteins genetics
Chromatin Immunoprecipitation
Gene Expression Profiling
Gene Expression Regulation, Plant
Isonicotinic Acids pharmacology
Oligonucleotide Array Sequence Analysis
Plants, Genetically Modified genetics
Plants, Genetically Modified metabolism
Protein Interaction Mapping
RNA, Plant metabolism
Stress, Physiological
Transcription Factors genetics
Transcriptional Activation
Triiodobenzoic Acids pharmacology
Two-Hybrid System Techniques
Xenobiotics pharmacology
Arabidopsis genetics
Arabidopsis Proteins metabolism
Promoter Regions, Genetic
Transcription Factors metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1040-4651
- Volume :
- 20
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- The Plant cell
- Publication Type :
- Academic Journal
- Accession number :
- 18984675
- Full Text :
- https://doi.org/10.1105/tpc.108.058974