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Use of Mycobacterium avium subsp. paratuberculosis specific coding sequences for serodiagnosis of bovine paratuberculosis.
- Source :
-
Veterinary microbiology [Vet Microbiol] 2009 Mar 30; Vol. 135 (3-4), pp. 313-9. Date of Electronic Publication: 2008 Sep 24. - Publication Year :
- 2009
-
Abstract
- In this study, the finished complete genome of Mycobacterium avium subsp. paratuberculosis (Map) was screened for specific coding sequences that could be very valuable in the design of a sensitive and specific Map detection serological assay. Eighty-seven Map-specific sequences were retained. Among these, three candidate antigens have been analysed for their serodiagnostic potential. These antigens were selected on the basis of their putative immunogenicity as predicted by in silico analysis. The antigens were cloned in Escherichia coli, expressed, and purified before testing in an antibody detection ELISA test, using a well characterized panel of 18 and 48 sera from Map infected and uninfected cattle, respectively. Two of these antigens, antigen 6 and MAP1637c, yielded in our conditions a sensitivity of 72% and 82%, respectively, for a specificity of 98%. It is particularly noticeable that, when probed with the same serum panel, the most widely used European paratuberculosis commercial seroassay (Pourquier test) yielded a sensitivity of 72% for a specificity of only 92%.
- Subjects :
- Animals
Antigens, Bacterial genetics
Bacterial Proteins genetics
Cattle
Cattle Diseases diagnosis
Cloning, Molecular
Enzyme-Linked Immunosorbent Assay
Escherichia coli genetics
Genome, Bacterial
Mass Spectrometry
Mycobacterium avium subsp. paratuberculosis isolation & purification
Polymerase Chain Reaction
Sensitivity and Specificity
Serologic Tests methods
Cattle Diseases microbiology
Mycobacterium avium subsp. paratuberculosis genetics
Paratuberculosis diagnosis
Subjects
Details
- Language :
- English
- ISSN :
- 0378-1135
- Volume :
- 135
- Issue :
- 3-4
- Database :
- MEDLINE
- Journal :
- Veterinary microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 18977614
- Full Text :
- https://doi.org/10.1016/j.vetmic.2008.09.065