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Phosphorylation of CAP-G is required for its chromosomal DNA localization during mitosis.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2008 Dec 19; Vol. 377 (3), pp. 1007-11. Date of Electronic Publication: 2008 Oct 31. - Publication Year :
- 2008
-
Abstract
- Condensin is a 5 subunit complex that plays an important role in the structure of chromosomes during mitosis. It is known that phosphorylation of condensin subunits by cdc2/cyclin B at the beginning of mitosis is important for condensin activity, but the sites of these phosphorylation events have not been identified nor has their role in regulating condensin function. Here we identify two threonine residues in the CAP-G subunit of condensin, threonines 308 and 332, that are targets of cdc2/cyclin B phosphorylation. Mutation of these threonines to alanines results in defects in CAP-G localization with chromosomes during mitosis. These results are the first to identify phosphorylation sites within the condensin complex that regulate condensin localization with chromosomal DNA.
- Subjects :
- Adenosine Triphosphatases genetics
Alanine genetics
Alanine metabolism
Amino Acid Sequence
Animals
CDC2 Protein Kinase metabolism
Cell Cycle Proteins genetics
Chromosomes, Human genetics
Cyclin B metabolism
DNA metabolism
DNA-Binding Proteins genetics
HeLa Cells
Humans
Mitosis
Molecular Sequence Data
Multiprotein Complexes genetics
Phosphorylation
Threonine genetics
Threonine metabolism
Xenopus
Adenosine Triphosphatases metabolism
Cell Cycle Proteins metabolism
Chromosomes, Human metabolism
DNA-Binding Proteins metabolism
Multiprotein Complexes metabolism
Mutation
Subjects
Details
- Language :
- English
- ISSN :
- 1090-2104
- Volume :
- 377
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 18977199
- Full Text :
- https://doi.org/10.1016/j.bbrc.2008.10.114