Back to Search
Start Over
Multipotency of clonal cells derived from swine periodontal ligament and differential regulation by fibroblast growth factor and bone morphogenetic protein.
- Source :
-
Journal of periodontal research [J Periodontal Res] 2009 Apr; Vol. 44 (2), pp. 238-47. Date of Electronic Publication: 2008 Oct 29. - Publication Year :
- 2009
-
Abstract
- Background and Objective: A blood supply is indispensable for the regeneration of damaged or lost periodontal ligament (PDL) tissue. Mesenchymal stem cell-like activity of cells derived from the PDL has been identified by their capacity to form fibrous and osseous tissue and cementum. However, it remains to be clarified whether the cells have an ability to build the capillary network of blood vessels. This study evaluated the potential of cells derived from the PDL to construct a blood vessel-like structure and examined how growth factors controlled the multipotency of the cells.<br />Material and Methods: The ability of a swine PDL fibroblast cell line, TesPDL3, to construct a blood vessel-like structure was evaluated on and in the self-assembling peptide scaffold, PuraMatrix(TM). In addition, the ability of the cells to form mineralized nodules was evaluated on type I collagen-coated plastic plates. In some cases, fibroblast growth factor (FGF)-2 and bone morphogenetic protein (BMP)-2 were added to these cultures. The status of the expression of vascular and osteoblastic cell-specific markers in the cells was evaluated using reverse transcriptase-polymerase chain reaction (RT-PCR), Western blotting and immunofluorescence analyses.<br />Results: The TesPDL3 cells not only formed mineralized nodules in response to BMP-2 stimulation but also constructed tube-like structures in response to FGF-2 stimulation. Intriguingly, FGF-2 inhibited the BMP-2-induced formation of mineralized nodules. Conversely, BMP-2 inhibited the FGF-2-induced formation of tube-like structures.<br />Conclusion: Periodontal ligament fibroblasts have the potential to differentiate not only into osteoblastic but also into vascular cell lineages. The destiny of the cells was reciprocally regulated by BMP-2 and FGF-2.
- Subjects :
- Animals
Bone Morphogenetic Protein 2 antagonists & inhibitors
Calcification, Physiologic physiology
Cell Culture Techniques
Cell Line
Clone Cells cytology
Clone Cells drug effects
Endothelium, Vascular cytology
Endothelium, Vascular drug effects
Fibroblast Growth Factor 2 antagonists & inhibitors
Fibroblasts cytology
Fibroblasts drug effects
Muscle, Smooth cytology
Muscle, Smooth drug effects
Neovascularization, Physiologic physiology
Osteoblasts cytology
Osteoblasts drug effects
Smad7 Protein physiology
Sus scrofa
Tissue Scaffolds
Bone Morphogenetic Protein 2 pharmacology
Calcification, Physiologic drug effects
Cell Differentiation drug effects
Fibroblast Growth Factor 2 pharmacology
Mesenchymal Stem Cells drug effects
Multipotent Stem Cells drug effects
Neovascularization, Physiologic drug effects
Periodontal Ligament cytology
Subjects
Details
- Language :
- English
- ISSN :
- 1600-0765
- Volume :
- 44
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of periodontal research
- Publication Type :
- Academic Journal
- Accession number :
- 18973516
- Full Text :
- https://doi.org/10.1111/j.1600-0765.2008.01140.x