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VirE1-Mediated Resistance to Crown Gall in Transgenic Arabidopsis thaliana.
- Source :
-
Phytopathology [Phytopathology] 2006 Jan; Vol. 96 (1), pp. 105-10. - Publication Year :
- 2006
-
Abstract
- ABSTRACT Crown gall disease, caused by Agrobacterium tumefaciens, remains a serious agricultural problem despite current biocontrol methods. Agrobacterium tumefaciens transfers single-stranded DNA (T-strands) into plant cells along with several virulence proteins, including a single-stranded DNA-binding protein (VirE2). In plant cells, T-strands are protected from nucleases and targeted to the nucleus by VirE2, which is essential for efficient transmission (transfer and integration) of T-strands. VirE1 is the secretory chaperone for VirE2; it prevents VirE2 from forming aggregates and from binding the T-strands in bacterial cells. Therefore, we hypothesized that sufficient quantities of VirE1 expressed in plant cells might block T-DNA transmission by preventing VirE2 from binding T-strands. Here we show that root explants from Arabidopsis thaliana plants that expressed virE1 formed 3.5-fold fewer tumors than roots from plants without virE1. Also, this resistance was specific for VirE2-mediated Agrobacterium transformation. Plants that have been genetically altered to resist crown gall may prove more effective than biological control.
Details
- Language :
- English
- ISSN :
- 0031-949X
- Volume :
- 96
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Phytopathology
- Publication Type :
- Academic Journal
- Accession number :
- 18944210
- Full Text :
- https://doi.org/10.1094/PHYTO-96-0105