Back to Search
Start Over
Quantitive cytokine mRNA expression profiles in the colonic mucosa of patients with steroid naïve ulcerative colitis during active and quiescent disease.
- Source :
-
Inflammatory bowel diseases [Inflamm Bowel Dis] 2009 Mar; Vol. 15 (3), pp. 328-34. - Publication Year :
- 2009
-
Abstract
- Background: Cytokines have validated roles in the immunopathogenesis of inflammatory bowel disease (IBD). This study was to investigate the expressions of tumor necrosis factor (TNF)-alpha, interleukin (IL)-6, IL-8, and IL-10 mRNAs in the colonic mucosa of patients with ulcerative colitis (UC) during active and quiescent UC.<br />Methods: At colonoscopy, biopsies were taken from inflamed and non-inflamed mucosa of patients with steroid-naive UC (n = 15), non-IBD inflammatory colitis controls (ICC, n = 6), and non-colitis controls (NCC, n = 14). The presence of extensive mononuclear cells and neutrophils infiltrate in the lamina propria, cryptitis, and epithelial damage defined an inflammatory lesion in the mucosa. Quantitative cytokine mRNA expressions in biopsies were measured by real-time polymerase chain reaction (PCR).<br />Results: Of 15 UC patients, 3 remitted with 5-aminosalicylate and 11 received granulocytapheresis; of these, 10 remitted. At baseline, IL-6, IL-8, TNF-alpha, and IL-10 mRNAs were high in inflamed mucosa compared with NCC (P < 0.01). In active UC, IL-6, IL-8 and IL-10 mRNAs were high compared with non-inflamed mucosa (P = 0.03, P = 0.03, P < 0.05, respectively). Both TNF-alpha mRNA (P = 0.03) and IL-6 mRNA (P = 0.04) were higher in UC compared with ICC. Even in non-inflamed mucosa, IL-8 and TNF-alpha mRNA expressions were high compared with NCC. Both IL-6 and IL-8 mRNAs decreased to normal levels after granulocytapheresis.<br />Conclusions: During active UC, all 4 cytokine mRNA levels were high; only IL-6 and IL-8 mRNAs decreased to normal levels during remission. IL-8 mRNA was high even at sites of endoscopically quiescent UC during active disease. Steroid naïve patients respond well to granulocytapheresis.
- Subjects :
- Administration, Oral
Adult
Anti-Inflammatory Agents, Non-Steroidal administration & dosage
Anti-Inflammatory Agents, Non-Steroidal therapeutic use
Biopsy
Colitis, Ulcerative metabolism
Colitis, Ulcerative therapy
Colon pathology
Colonoscopy
Cytokines biosynthesis
Dose-Response Relationship, Drug
Female
Follow-Up Studies
Granulocytes
Humans
Interleukin-6 biosynthesis
Interleukin-6 genetics
Interleukin-8 biosynthesis
Interleukin-8 genetics
Intestinal Mucosa metabolism
Intestinal Mucosa pathology
Leukapheresis methods
Male
Mesalamine administration & dosage
Middle Aged
Polymerase Chain Reaction
Prognosis
RNA, Messenger biosynthesis
Remission Induction methods
Retrospective Studies
Tumor Necrosis Factor-alpha biosynthesis
Tumor Necrosis Factor-alpha genetics
Young Adult
Colitis, Ulcerative genetics
Colon metabolism
Cytokines genetics
Drug Resistance genetics
Gene Expression Regulation
Mesalamine therapeutic use
RNA, Messenger genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1536-4844
- Volume :
- 15
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Inflammatory bowel diseases
- Publication Type :
- Academic Journal
- Accession number :
- 18942752
- Full Text :
- https://doi.org/10.1002/ibd.20759