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ElrA and AUF1 differentially bind cyclin B2 mRNA.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2008 Dec 12; Vol. 377 (2), pp. 653-657. Date of Electronic Publication: 2008 Oct 16. - Publication Year :
- 2008
-
Abstract
- In Xenopus embryos, maternal cyclins drive the first 12 cell divisions after which several cyclins are terminally degraded, including cyclin B2. Cyclin B2 disappearance is due to transcription-mediated mRNA deadenylation at the midblastula transition, when transcription initiates and the cell cycle lengthens. To further define the mechanism, we characterized proteins capable of binding cyclin B2 3'UTR. We show that ElrA and AUF1 compete for binding to regions containing cytoplasmic polyadenylation elements (CPEs), with AUF1 binding increasing at the midblastula transition. Deletion of both CPEs abrogates polyadenylation but has no effect on deadenylation or binding of ElrA or AUF1. Overexpression of ElrA or AUF1 does not alter cyclin B2 mRNA stability. These results show that ElrA and AUF1 bind to cyclin B2 mRNA independent of CPEs and function by binding other elements.
- Subjects :
- Animals
Binding, Competitive
Cyclin B2
Heterogeneous Nuclear Ribonucleoprotein D0
Heterogeneous-Nuclear Ribonucleoprotein D genetics
Polyadenylation
Ribonucleoproteins genetics
Xenopus Proteins genetics
Xenopus laevis genetics
3' Untranslated Regions metabolism
Cyclin B biosynthesis
Heterogeneous-Nuclear Ribonucleoprotein D metabolism
RNA Stability
Ribonucleoproteins metabolism
Xenopus Proteins metabolism
Xenopus laevis metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1090-2104
- Volume :
- 377
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 18930026
- Full Text :
- https://doi.org/10.1016/j.bbrc.2008.10.029