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Hematopoietic cell activation in the subventricular zone after Theiler's virus infection.
- Source :
-
Journal of neuroinflammation [J Neuroinflammation] 2008 Oct 15; Vol. 5, pp. 44. Date of Electronic Publication: 2008 Oct 15. - Publication Year :
- 2008
-
Abstract
- Background: The periventricular subventricular zone (SVZ) contains stem cells and is an area of active neurogenesis and migration. Since inflammation can reduce neurogenesis, we tested whether Theiler's murine encephalomyelitis virus (TMEV) induces inflammation and reduces neurogenesis in the SVZ.<br />Methods: We performed immmunohistochemistry for the hematopoietic cell marker CD45 throughout the central nervous system and then examined neuroblasts in the SVZ.<br />Results: CD45+ activation (inflammation) occurred early in the forebrain and preceded cerebellar and spinal cord inflammation. Inflammation in the brain was regionally stochastic except for the SVZ and surrounding periventricular regions where it was remarkably pronounced and consistent. In preclinical mice, SVZ neuroblasts emigrated into inflamed periventricular regions. The number of proliferating phoshpohistone3+ cells and Doublecortin+ (Dcx) SVZ neuroblasts was overall unaffected during the periods of greatest inflammation. However the number of Dcx+ and polysialylated neural cell adhesion molecule (PSA-NCAM+) SVZ neuroblasts decreased only after periventricular inflammation abated.<br />Conclusion: Our results suggest that after TMEV infection, the SVZ may mount an attempt at neuronal repair via emigration, a process dampened by decreases in neuroblast numbers.
- Subjects :
- Animals
Biomarkers analysis
Biomarkers metabolism
Cardiovirus Infections physiopathology
Cell Lineage immunology
Cell Movement immunology
Cell Proliferation
Disease Models, Animal
Doublecortin Domain Proteins
Doublecortin Protein
Encephalitis physiopathology
Female
Hematopoietic Stem Cells virology
Histones metabolism
Lateral Ventricles
Mice
Microtubule-Associated Proteins metabolism
Multiple Sclerosis immunology
Multiple Sclerosis physiopathology
Nerve Regeneration immunology
Neuronal Plasticity immunology
Neurons cytology
Neurons immunology
Neuropeptides metabolism
Prosencephalon immunology
Prosencephalon pathology
Prosencephalon physiopathology
Cardiovirus Infections immunology
Encephalitis immunology
Hematopoietic Stem Cells immunology
Leukocyte Common Antigens immunology
Neurogenesis immunology
Theilovirus immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1742-2094
- Volume :
- 5
- Database :
- MEDLINE
- Journal :
- Journal of neuroinflammation
- Publication Type :
- Academic Journal
- Accession number :
- 18922161
- Full Text :
- https://doi.org/10.1186/1742-2094-5-44