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Differential suppression of host cell protein synthesis and mRNA levels in herpes simplex virus-infected endothelial cells.
- Source :
-
Virus research [Virus Res] 1991 May; Vol. 19 (2-3), pp. 209-21. - Publication Year :
- 1991
-
Abstract
- Earlier studies from this laboratory have shown that infection of vascular cells with herpes simplex virus 1 or 2 (HSV-1, HSV-2) results in the differential suppression of extracellular matrix proteins including fibronectin (FN), type IV collagen, thrombospondin (TSP) and Factor VIII von Willebrand protein. The present study was designed to determine whether a correlation exists between suppression of synthesis of specific proteins and their mRNA levels. We have measured the steady-state levels of mRNAs for several extracellular matrix proteins (type IV collagen, FN and TSP) and two intracellular proteins (actin and tubulin) in human endothelial cells (EC) following HSV-1 infection. The results show that during the first 5 h post-infection, when there is a rapid decrease in the synthesis of extracellular matrix proteins, the steady-state levels of the corresponding mRNAs remain relatively high, but progressively decline to levels of less than 20% by 13 h post-infection. These findings suggest that in the early hours post-infection there is an alteration in the translatability of the hybridizable message followed by degradation in the later hours.
- Subjects :
- Cells, Cultured
Endothelium, Vascular metabolism
Endothelium, Vascular microbiology
Extracellular Matrix Proteins biosynthesis
Extracellular Matrix Proteins genetics
Herpes Simplex genetics
Humans
Proteins genetics
RNA, Messenger genetics
Time Factors
Herpes Simplex metabolism
Protein Biosynthesis
RNA, Messenger metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0168-1702
- Volume :
- 19
- Issue :
- 2-3
- Database :
- MEDLINE
- Journal :
- Virus research
- Publication Type :
- Academic Journal
- Accession number :
- 1891960
- Full Text :
- https://doi.org/10.1016/0168-1702(91)90047-y