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Effect of aging, caloric restriction, and uncoupling protein 3 (UCP3) on mitochondrial proton leak in mice.
- Source :
-
Experimental gerontology [Exp Gerontol] 2008 Dec; Vol. 43 (12), pp. 1069-76. Date of Electronic Publication: 2008 Sep 30. - Publication Year :
- 2008
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Abstract
- Mitochondrial proton leak may modulate reactive oxygen species (ROS) production and play a role in aging. The purpose of this study was to determine proton leak across the life span in skeletal mitochondria from calorie-restricted and UCP2/3 overexpressing mice. Proton leak in isolated mitochondria and markers of oxidative stress in whole tissue were measured in female C57BL/6J mice fed ad-libitum (WT-Control) or a 30% calorie-restricted (WT-CR) diet, and in mice overexpressing UCP2 and UCP3 (Positive-TG), their non-overexpressing littermates (Negative-TG) and UCP3 knockout mice (UCP3KO). Proton leak in WT-CR mice was lower than that of control mice at 8 and 26 months of age. The Positive-TG mice had greater proton leak than the Negative-TG and UCP3KO mice at 8 months of age, but this difference disappeared by 19 and 26 months. Lipid peroxidation was generally lower in WT-CR vs. WT-Control mice and UCP3KO mice had greater concentrations of T-BARS (thiobarbituric acid reactive substances, a measure of lipid peroxidation) than did Positive-TG and Negative-TG. The results of this study indicate that sustained increases in muscle mitochondrial proton leak are not responsible for alterations in life span with calorie restriction or UCP3 overexpression in mice. However, UCP3 may contribute to the actions of CR through mechanisms distinct from increasing basal proton leak.
- Subjects :
- Animals
Gangliosides
Lipid Peroxidation physiology
Mice
Mice, Knockout
Muscle, Skeletal metabolism
Oxidative Stress physiology
Protons
Uncoupling Protein 3
Aging physiology
Caloric Restriction mortality
Ion Channels metabolism
Mitochondria, Muscle metabolism
Mitochondrial Proteins metabolism
Reactive Oxygen Species metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1873-6815
- Volume :
- 43
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Experimental gerontology
- Publication Type :
- Academic Journal
- Accession number :
- 18852040
- Full Text :
- https://doi.org/10.1016/j.exger.2008.09.010