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Whole lymphoma B cells allow efficient cross-presentation of antigens by dendritic cells.
Whole lymphoma B cells allow efficient cross-presentation of antigens by dendritic cells.
- Source :
-
Cytotherapy [Cytotherapy] 2008; Vol. 10 (6), pp. 642-9. - Publication Year :
- 2008
-
Abstract
- Background: In order to compensate for the paucity of defined tumor antigens (Ag) in non-Hodgkin's lymphomas, a promising approach might be the use of whole tumor cells as a source of tumor Ag to pulse antigen-presenting cells (APC). However, it is not presently known how the tumor cells should be delivered to APC to optimize the cross-presentation of tumor Ag to anti-tumor CD8 T cells. We aimed to compare CD20-opsonized, apoptotic and necrotic human tumor cells for their capacity to induce endocytosis and cross-presentation of tumor-associated Ag by dendritic cells (DC) or macrophages.<br />Methods: Endocytosis of human tumor-derived material by macrophages or DC was monitored by flow cytometry. We used a previously described influenza model and studied cross-presentation of viral Ag as cellular surrogate tumor-associated Ag by APC after endocytosis of lymphoma B cells treated by inactivated influenza virus.<br />Results: Optimal endocytosis was obtained when tumor cells were opsonized by an anti-CD20 antibody and, as expected, macrophages were more phagocytic than DC. However, Ag from opsonized, apoptotic and live cells, but not from necrotic lymphoma cells, were efficiently cross-presented by DC but not by macrophages.<br />Discussion: We have developed a new model with human primary lymphoma cells to study the cross-presentation of tumor-associated Ag by APC. The results we have obtained support the use of whole lymphoma cells from patients to pulse DC to induce an anti-tumor immune response.
- Subjects :
- Antibodies, Monoclonal pharmacology
Antibodies, Monoclonal, Murine-Derived
Antigen Presentation drug effects
Antigen Presentation immunology
Antigens, CD20 immunology
Antigens, Neoplasm drug effects
Antigens, Neoplasm immunology
Antigens, Viral immunology
CD8-Positive T-Lymphocytes metabolism
Dendritic Cells cytology
Dendritic Cells virology
Humans
Immunologic Factors pharmacology
Lymphocyte Activation immunology
Macrophages cytology
Orthomyxoviridae immunology
Rituximab
CD8-Positive T-Lymphocytes immunology
Cross-Priming immunology
Dendritic Cells immunology
Lymphoma, B-Cell immunology
Macrophages immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1477-2566
- Volume :
- 10
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Cytotherapy
- Publication Type :
- Academic Journal
- Accession number :
- 18836919
- Full Text :
- https://doi.org/10.1080/14653240802317647