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Whole lymphoma B cells allow efficient cross-presentation of antigens by dendritic cells.

Whole lymphoma B cells allow efficient cross-presentation of antigens by dendritic cells.

Authors :
Manches O
Lui G
Molens JP
Sotto JJ
Chaperot L
Plumas J
Source :
Cytotherapy [Cytotherapy] 2008; Vol. 10 (6), pp. 642-9.
Publication Year :
2008

Abstract

Background: In order to compensate for the paucity of defined tumor antigens (Ag) in non-Hodgkin's lymphomas, a promising approach might be the use of whole tumor cells as a source of tumor Ag to pulse antigen-presenting cells (APC). However, it is not presently known how the tumor cells should be delivered to APC to optimize the cross-presentation of tumor Ag to anti-tumor CD8 T cells. We aimed to compare CD20-opsonized, apoptotic and necrotic human tumor cells for their capacity to induce endocytosis and cross-presentation of tumor-associated Ag by dendritic cells (DC) or macrophages.<br />Methods: Endocytosis of human tumor-derived material by macrophages or DC was monitored by flow cytometry. We used a previously described influenza model and studied cross-presentation of viral Ag as cellular surrogate tumor-associated Ag by APC after endocytosis of lymphoma B cells treated by inactivated influenza virus.<br />Results: Optimal endocytosis was obtained when tumor cells were opsonized by an anti-CD20 antibody and, as expected, macrophages were more phagocytic than DC. However, Ag from opsonized, apoptotic and live cells, but not from necrotic lymphoma cells, were efficiently cross-presented by DC but not by macrophages.<br />Discussion: We have developed a new model with human primary lymphoma cells to study the cross-presentation of tumor-associated Ag by APC. The results we have obtained support the use of whole lymphoma cells from patients to pulse DC to induce an anti-tumor immune response.

Details

Language :
English
ISSN :
1477-2566
Volume :
10
Issue :
6
Database :
MEDLINE
Journal :
Cytotherapy
Publication Type :
Academic Journal
Accession number :
18836919
Full Text :
https://doi.org/10.1080/14653240802317647