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Pharmacokinetic study of clazuril (Appertex) in eggs and plasma from laying hens after single or multiple treatments, using a new HPLC method for detection.
- Source :
-
British poultry science [Br Poult Sci] 2008 Sep; Vol. 49 (5), pp. 609-18. - Publication Year :
- 2008
-
Abstract
- 1. Anticoccidials are widely used as food additives to prevent and treat coccidiosis. They are licensed for use in a prescribed concentration and during a specific time interval with broilers and pullets, but not for laying hens. 2. This study was set up to develop a new high pressure liquid chromatography (HPLC) method to detect clazuril (CZ: (+/-)-2-chloro-alpha-(4-chlorophenyl)-4-(4,5-dihydro-3,5-dioxo-1,2,4-triazin-2(3H)-yl)-benzeneacetonitrile) in egg yolk and albumen and in plasma; to investigate both the presence of residues of CZ in eggs and its pharmacokinetic behaviour in laying hens. 3. A single oral dose (3 mg/kg BW) and multiple oral doses (3 mg/kg BW for 5 d) were investigated. The analytical method gave very good recovery (64 to 74%) in the three different matrices (yolk, albumen and plasma); precision and accuracy were within 11%. 4. After a single dose no residue was detected in eggs collected for up to 10 d, while following multiple dose treatment, CZ residues were detected until 10 d after the end of treatment. The concentration of the drug was higher in yolk than in albumen with a maximum ratio of 10 : 1. 5. Pharmacokinetics of CZ in laying hens after a single dose showed a detectable concentration of the drug up to 24 h. It reached a steady state after the third administration in multiple dosing. 6. Although further studies are necessary, these results indicate that a single oral dose of CZ could be used as an anticoccidial for laying hens due to the lack of residues in eggs.
- Subjects :
- Acetonitriles chemistry
Albumins chemistry
Animal Feed
Animals
Area Under Curve
Coccidiostats blood
Coccidiostats chemistry
Coccidiostats pharmacokinetics
Drug Administration Schedule
Drug Residues analysis
Egg Yolk chemistry
Female
Half-Life
Molecular Structure
Reproducibility of Results
Triazines chemistry
Acetonitriles blood
Acetonitriles pharmacokinetics
Chickens metabolism
Chromatography, High Pressure Liquid veterinary
Eggs analysis
Triazines blood
Triazines pharmacokinetics
Subjects
Details
- Language :
- English
- ISSN :
- 1466-1799
- Volume :
- 49
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- British poultry science
- Publication Type :
- Academic Journal
- Accession number :
- 18836908
- Full Text :
- https://doi.org/10.1080/00071660802294566