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Analysis of the pSK1 replicon, a prototype from the staphylococcal multiresistance plasmid family.
- Source :
-
Microbiology (Reading, England) [Microbiology (Reading)] 2008 Oct; Vol. 154 (Pt 10), pp. 3084-3094. - Publication Year :
- 2008
-
Abstract
- Multidrug-resistant staphylococci often harbour plasmids that carry genes conferring resistance to several antimicrobial compounds. Many of these multiresistance plasmids appear to utilize a related theta-type replication system for which multiresistance plasmid pSK1 serves as a prototype. Essential pSK1 replication elements were identified by cloning segments of the replication region and testing the resulting plasmids for replication proficiency. An iterated region within rep and a DNA segment of up to 68 bp upstream of the rep promoter were both found to be essential for origin activity. The Rep protein was overexpressed as a 6xHis-tagged C-terminal fusion protein and was shown to bind in vitro to four Rep boxes located within the rep coding region. Inactivation of a divergently oriented promoter upstream of rep, designated P(rnaI), resulted in an elevated plasmid copy number. Comparative analyses suggest that the replication systems of many staphylococcal multiresistance plasmids share a similar genetic organization and utilize an antisense-RNA-mediated regulatory mechanism for copy number control.
- Subjects :
- Bacterial Proteins genetics
Base Sequence
DNA Replication
DNA, Bacterial genetics
DNA-Binding Proteins genetics
Escherichia coli genetics
Gene Dosage
Gene Expression Regulation, Bacterial
Molecular Sequence Data
Mutation
Promoter Regions, Genetic
RNA, Antisense genetics
RNA, Bacterial genetics
Transcription Initiation Site
Drug Resistance, Multiple, Bacterial genetics
Plasmids genetics
Replicon
Staphylococcus aureus genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1350-0872
- Volume :
- 154
- Issue :
- Pt 10
- Database :
- MEDLINE
- Journal :
- Microbiology (Reading, England)
- Publication Type :
- Academic Journal
- Accession number :
- 18832314
- Full Text :
- https://doi.org/10.1099/mic.0.2008/017418-0