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Isolation and characterization of the CRIPTO autosomal gene and its X-linked related sequence.
- Source :
-
American journal of human genetics [Am J Hum Genet] 1991 Sep; Vol. 49 (3), pp. 555-65. - Publication Year :
- 1991
-
Abstract
- We have previously reported on the identification of a cDNA clone encoding a novel human growth factor, named "CRIPTO," that is abundantly expressed in undifferentiated human NTERA-2 clone D1 (NT2/D1) and mouse (F9) teratocarcinoma cells. We now report the organization and nucleotide sequence of two related genomic sequences. One (CR-1) corresponds to the structural gene encoding the human CRIPTO protein expressed in the undifferentiated human teratocarcinoma cells, and the other (CR-3) corresponds to a complete copy of the mRNA containing seven base substitutions in the coding region representing both silent and replacement substitutions. The 440 bp 5' to the CAP site of CR-1 are preserved in CR-3. CR-1 maps to chromosome 3, and CR-3 maps to Xq21-q22. Southern blot analysis reveals that multiple CRIPTO-related DNA sequences are present in the human as well as in the mouse genome.
- Subjects :
- Amino Acid Sequence
Animals
Base Sequence
Blotting, Southern
Cell Line
Chromosome Mapping
GPI-Linked Proteins
Humans
Intercellular Signaling Peptides and Proteins
Mice
Molecular Sequence Data
Multigene Family genetics
Mutation genetics
Tumor Cells, Cultured
Chromosomes, Human, Pair 3
Epidermal Growth Factor
Growth Substances genetics
Membrane Glycoproteins
Neoplasm Proteins genetics
X Chromosome
Subjects
Details
- Language :
- English
- ISSN :
- 0002-9297
- Volume :
- 49
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- American journal of human genetics
- Publication Type :
- Academic Journal
- Accession number :
- 1882841