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Hormonal regulation of oil accumulation in Brassica seeds: metabolism and biological activity of ABA, 7'-, 8'- and 9'-hydroxy ABA in microspore derived embryos of B. napus.
- Source :
-
Phytochemistry [Phytochemistry] 2008 Nov; Vol. 69 (15), pp. 2678-88. Date of Electronic Publication: 2008 Sep 26. - Publication Year :
- 2008
-
Abstract
- Developing seeds of Brassica napus contain significant levels of ABA and products of oxidation at the 7'- and 9'-methyl groups of ABA, 7'- and 9'-hydroxy ABA, as well stable products of oxidation of the 8'-methyl group, phaseic acid and dihydrophaseic acid. To probe the biological roles of the initially formed hydroxylated compounds, we have compared the effects of supplied ABA and the hydroxylated metabolites in regulating oil synthesis in microspore-derived embryos of B. napus, cv Hero that accumulate long chain fatty acids. Uptake into the embryos and metabolism of each of the hormone metabolites was studied by using deuterium labeled analogs. Supplied ABA, which was rapidly metabolized, induced expression of oleosin and fatty acid elongase genes and increased the accumulation of triacylglycerols and very long chain fatty acids. The metabolites 7'- and 9'-hydroxy ABA had similar effects, with the 9'-hydroxy ABA having even greater activity than ABA. The principal catabolite of ABA, 8'-hydroxy ABA, also had hormonal activity and led to increased oil synthesis but induced the genes weakly. These results indicate that all compounds tested could be involved in lipid synthesis in B. napus, and may have hormonal roles in other ABA-regulated processes.
- Subjects :
- Abscisic Acid pharmacology
Acetyltransferases metabolism
Brassica napus embryology
Brassica napus genetics
Fatty Acid Elongases
Fatty Acids, Monounsaturated metabolism
Gene Expression Regulation, Plant drug effects
Hormones pharmacology
Plant Proteins genetics
Seeds embryology
Seeds genetics
Triglycerides metabolism
Abscisic Acid metabolism
Brassica napus metabolism
Hormones metabolism
Oils metabolism
Seeds metabolism
Spores metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0031-9422
- Volume :
- 69
- Issue :
- 15
- Database :
- MEDLINE
- Journal :
- Phytochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 18823922
- Full Text :
- https://doi.org/10.1016/j.phytochem.2008.08.010