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Proteome analysis of multidrug resistance of human oral squamous carcinoma cells using CD147 silencing.
- Source :
-
Journal of proteome research [J Proteome Res] 2008 Nov; Vol. 7 (11), pp. 4784-91. Date of Electronic Publication: 2008 Sep 25. - Publication Year :
- 2008
-
Abstract
- There is a correlation between the multidrug-resistance (MDR) of cancer cells and their enhanced invasive or metastatic potential. We studied the expression of CD147, a plasma membrane glycoprotein that plays a key role in tumor metastasis by stimulating the production of matrix metalloproteinases (MMPs), in sensitive human oral squamous KB and MDR derivative KB/V cells. Reverse transcription-PCR and flow cytometric analysis revealed that KB/V cells expressed CD147 at significantly higher levels than their parental KB cells. Using stable RNA interference, we succeeded in establishing a CD147 knock-down KB/V cell line (KB/VsiCD147). MTT colorimetric assay showed an increase in the chemosensitivity to vincristine (VCR), all transretinoic acid (ATRA), taxol, and 5-fluorouracil (5-Fu) of KB/VsiCD147 cells. Proteome analysis of KB, KB/V, and KB/VsiCD147 cell lines identified 21 differently expressed proteins. The enhanced expression of representative active proteins, GRP75 and CyPA, was confirmed by Western blotting and RT-PCR. In addition, pretreatment of KB/V cells with a CyPA-binding immunosuppressive drug, cyclosporine A (CsA), enhanced their chemosensitivity to VCR and 5-Fu. We document an abundance of molecules that interact with CD147 in the MDR of human oral squamous carcinoma cells. Additional studies are needed to investigate these novel target proteins of CD147.
- Subjects :
- Carcinoma, Squamous Cell pathology
Cell Line, Tumor
Coloring Agents metabolism
Formazans metabolism
Humans
Proteomics methods
RNA Interference
RNA, Small Interfering metabolism
Tetrazolium Salts metabolism
Transfection
Basigin genetics
Carcinoma, Squamous Cell genetics
Drug Resistance, Multiple genetics
Drug Resistance, Neoplasm genetics
Proteome analysis
Subjects
Details
- Language :
- English
- ISSN :
- 1535-3893
- Volume :
- 7
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Journal of proteome research
- Publication Type :
- Academic Journal
- Accession number :
- 18816083
- Full Text :
- https://doi.org/10.1021/pr800355b