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SigF controls carotenoid pigment production and affects transformation efficiency and hydrogen peroxide sensitivity in Mycobacterium smegmatis.
- Source :
-
Journal of bacteriology [J Bacteriol] 2008 Dec; Vol. 190 (23), pp. 7859-63. Date of Electronic Publication: 2008 Sep 19. - Publication Year :
- 2008
-
Abstract
- Carotenoids are complex lipids that are known for acting against photodynamic injury and free radicals. We demonstrate here that sigma(F) is required for carotenoid pigment production in Mycobacterium smegmatis. We further show that a sigF mutant exhibits a transformation efficiency 10(4)-fold higher than that of the parental strain, suggesting that sigma(F) regulates the production of components affecting cell wall permeability. In addition, a sigF mutant showed an increased sensitivity to hydrogen peroxide. An in silico search of the M. smegmatis genome identified a number of SigF consensus sites, including sites upstream of the carotenoid synthesis locus, which explains its SigF regulation.
- Subjects :
- DNA Transposable Elements genetics
Gene Expression Regulation, Bacterial
Mutagenesis, Insertional
Bacterial Proteins metabolism
Carotenoids biosynthesis
Hydrogen Peroxide pharmacology
Mycobacterium smegmatis drug effects
Mycobacterium smegmatis metabolism
Oxidants pharmacology
Sigma Factor metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1098-5530
- Volume :
- 190
- Issue :
- 23
- Database :
- MEDLINE
- Journal :
- Journal of bacteriology
- Publication Type :
- Academic Journal
- Accession number :
- 18805974
- Full Text :
- https://doi.org/10.1128/JB.00714-08