Back to Search
Start Over
Low-vitamin E diet exacerbates calcium oxalate crystal formation via enhanced oxidative stress in rat hyperoxaluric kidney.
- Source :
-
American journal of physiology. Renal physiology [Am J Physiol Renal Physiol] 2009 Jan; Vol. 296 (1), pp. F34-45. Date of Electronic Publication: 2008 Sep 17. - Publication Year :
- 2009
-
Abstract
- Vitamin E was previously reported to reduce calcium oxalate (CaOx) crystal formation. This study explored whether vitamin E deficiency affects intrarenal oxidative stress and accelerates crystal deposition in hyperoxaluria. The control (C) group of rats received a standard diet and drinking water, while the experimental groups received 0.75% ethylene glycol (EG) in drinking water for 42 days. Of the latter, one group received a standard diet (EG group), one received a low-vitamin E (LE) diet (EG+LE group), and the last received an LE diet with vitamin E supplement (4 mg) (EG+LE+E group). The C+LE and C+LE+E groups were the specific controls for the last two experimental groups, respectively. In a separate experiment, EG and EG+LE rats were studied on days 3-42 to examine the temporal relationship between oxidative change and crystal formation. Urinary biochemistry and activity/levels of antioxidative and oxidative enzymes in glomeruli and tubulointerstitial specimens (TIS) were examined. In EG rats, CaOx crystal accumulation was associated with low antioxidative enzyme activity in TIS and with increased oxidative enzyme expression in glomeruli. In the EG+LE group, marked changes in antioxidative and oxidative enzyme levels were seen and correlated with massive CaOx deposition and tubular damage. The increased oxidative stress seen with EG+LE treatment was largely reversed by vitamin E supplementation. A temporal study showed that decrease in antioxidative defense and increased free radical formation in the EG+LE group occurred before crystal deposition. This study shows that low vitamin E disrupts the redox balance and causes cell death, thereby favoring crystal formation.
- Subjects :
- Animals
Crystallization
Dietary Supplements
Disease Models, Animal
Ethylene Glycol
Free Radicals metabolism
Hyperoxaluria chemically induced
Hyperoxaluria physiopathology
Kidney physiopathology
Male
Membrane Glycoproteins metabolism
NADPH Oxidase 2
NADPH Oxidases metabolism
Oxidative Stress drug effects
Rats
Rats, Wistar
Superoxides metabolism
Vitamin E metabolism
Vitamin E pharmacology
Xanthine Oxidase metabolism
Calcium Oxalate metabolism
Hyperoxaluria metabolism
Kidney metabolism
Oxidative Stress physiology
Vitamin E Deficiency metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1931-857X
- Volume :
- 296
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- American journal of physiology. Renal physiology
- Publication Type :
- Academic Journal
- Accession number :
- 18799548
- Full Text :
- https://doi.org/10.1152/ajprenal.90309.2008