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Use of liposomes as membrane models to evaluate the contribution of drug-membrane interactions to antioxidant properties of etodolac.
- Source :
-
Redox report : communications in free radical research [Redox Rep] 2008; Vol. 13 (5), pp. 225-36. - Publication Year :
- 2008
-
Abstract
- This work stresses the need to combine antioxidant assays and drug-membrane interaction studies to describe more accurately the antioxidant profile of non-steroidal anti-inflammatory drugs (NSAIDs). Different experiments performed in liposomes and aqueous solution were compared and used to evaluate the protective effect of etodolac in lipid peroxidation. Lipid peroxidation was induced by the peroxyl radical (ROO*) derived from 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH) and hydroxyl radical (HO*) generated by the Fenton reaction and was assessed by the fluorescence intensity decay of three fluorescence probes with distinct lipophilic properties--fluorescein; hexadecanoyl aminofluorescein (HDAF) and diphenylhexatriene propionic acid (DPHPA). Membrane fluidity changes due to lipid peroxidation were also evaluated by steady-state anisotropy measurements. Interactions of etodolac with lipid bilayers were evaluated by membrane zeta-potential measurements. Results indicate a drug location near the membrane surface and show that etodolac can scavenge the radicals studied but to a variable extent, depending on the assayed media and reactive species. The use of different probes and liposomes as membrane mimetic systems allowed us to conclude that membrane lipoperoxidation is not only related to the scavenging characteristics of the antioxidants, but also to their ability to interact with lipid bilayers.
- Subjects :
- Anisotropy
Chromans pharmacology
Diphenylhexatriene analogs & derivatives
Diphenylhexatriene chemistry
Fluorescein chemistry
Fluoresceins chemistry
Fluorescent Dyes chemistry
Free Radical Scavengers pharmacology
Membrane Fluidity drug effects
Membrane Potentials drug effects
Antioxidants pharmacology
Etodolac pharmacology
Lipid Peroxidation drug effects
Unilamellar Liposomes metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1743-2928
- Volume :
- 13
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Redox report : communications in free radical research
- Publication Type :
- Academic Journal
- Accession number :
- 18796242
- Full Text :
- https://doi.org/10.1179/135100008X308939