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Structure and anti-dengue virus activity of sulfated polysaccharide from a marine alga.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2008 Nov 07; Vol. 376 (1), pp. 91-5. Date of Electronic Publication: 2008 Aug 30. - Publication Year :
- 2008
-
Abstract
- A sulfated polysaccharide, named fucoidan, from the marine alga Cladosiphon okamuranus is comprised of carbohydrate units containing glucuronic acid and sulfated fucose residues. Here we found this compound potently inhibits dengue virus type 2 (DEN2) infection. Viral infection was inhibited when DEN2, but not other serotypes, was pretreated with fucoidan. A carboxy-reduced fucoidan derivative in which glucuronic acid was converted to glucose did not inhibit viral infection. Elimination of the sulfated function group from fucoidan significantly attenuated the inhibitory activity on DEN2 infection with <1% fucoidan. DEN2 particles bound exclusively to fucoidan, indicating that fucoidan interacts directly with envelope glycoprotein (EGP) on DEN2. Structure-based analysis suggested that Arg323 of DEN2 EGP, which is conformationally proximal to one of the putative heparin binding residues, Lys310, is critical for the interaction with fucoidan. In conclusion, both the sulfated group and glucuronic acid of fucoidan account for the inhibition of DEN2 infection.
- Subjects :
- Amino Acid Sequence
Animals
Antiviral Agents chemistry
Antiviral Agents metabolism
Cell Line
Cricetinae
Dengue Virus genetics
Dengue Virus metabolism
Molecular Sequence Data
Polysaccharides chemistry
Polysaccharides metabolism
Protein Conformation
Protein Structure, Tertiary
Structure-Activity Relationship
Viral Envelope Proteins chemistry
Viral Envelope Proteins genetics
Viral Envelope Proteins metabolism
Antiviral Agents pharmacology
Dengue Virus drug effects
Phaeophyceae chemistry
Polysaccharides pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1090-2104
- Volume :
- 376
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 18762172
- Full Text :
- https://doi.org/10.1016/j.bbrc.2008.08.100