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On-chip detection of protein glycosylation based on energy transfer between nanoparticles.
- Source :
-
Biosensors & bioelectronics [Biosens Bioelectron] 2009 Jan 01; Vol. 24 (5), pp. 1189-94. Date of Electronic Publication: 2008 Jul 18. - Publication Year :
- 2009
-
Abstract
- We describe a chip-based method to detect protein glycosylation based on the energy transfer between quantum dots (QDs) and gold nanoparticles (AuNPs). Our assay system relies on modulations in the energy transfer between the nanoparticles on a surface. The photoluminescence (PL) of lectin-coated QDs (energy donor) immobilized on a glass slide is quenched by carbohydrate-coated AuNPs (energy acceptor), and the presence of the glycoprotein causes the increase of the PL of QDs. As a proof-of-concept, Concanavalin A-coated QDs (ConA-QDs) and dextran-coated AuNPs (Dex-AuNPs) were used to detect the mannosylated proteins. As a result, the PL intensity of QDs was found to be linearly correlated with the concentration and the number of glycan moiety of the glycoprotein. We anticipated that our simple assay system will find applications for the analysis of glycoproteins with high selectivity and sensitivity in a high-throughput manner.
- Subjects :
- Biosensing Techniques methods
Equipment Design
Equipment Failure Analysis
Fluorescence Resonance Energy Transfer methods
Glycoproteins analysis
Glycosylation
Reproducibility of Results
Sensitivity and Specificity
Biosensing Techniques instrumentation
Fluorescence Resonance Energy Transfer instrumentation
Glycoproteins chemistry
Nanoparticles chemistry
Protein Array Analysis instrumentation
Quantum Dots
Subjects
Details
- Language :
- English
- ISSN :
- 1873-4235
- Volume :
- 24
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Biosensors & bioelectronics
- Publication Type :
- Academic Journal
- Accession number :
- 18722763
- Full Text :
- https://doi.org/10.1016/j.bios.2008.07.012