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[Characterizing the CD3 epsilon chain from the New World primate Aotus nancymaae].
- Source :
-
Biomedica : revista del Instituto Nacional de Salud [Biomedica] 2008 Jun; Vol. 28 (2), pp. 262-70. - Publication Year :
- 2008
-
Abstract
- Introduction: The T-cell receptor (TCR)-associated complex, CD3 (d, g, e) and z-chains are essential transmembrane proteins for signal transduction during T cell activation and immune response, as well as during thymocyte development.<br />Objective: This work established the CD3epsilon-chain primary structure for the New World owl monkey Aotus nancymaae.<br />Materials and Methods: Total RNA was isolated from peripheral blood mononuclear cells; CD3epsilon molecule was amplified, cloned and sequenced.<br />Results: The CD3epsilon amino acid sequence was deduced for the owl monkey Aotus nancymaae.> It has an identity for nucleotide and amino acid sequences with the human counterpart of 84% and 76%, respectively. As described in other species, the Aotus CD3-e molecule is very variable in the extracellular region and greatly conserved in the intracellular domain. Even though high variability occurs in the CD3epsilon-extracellular domain, the subregions involved in ectodomain folding are conserved.<br />Conclusions: The primary structure suggested that the Aotus protein has a functional role similar to that of humans, and that the initial T-cell activation steps are also similar. However, the great variation observed in CD3epsilon-extracellular region in humans in contrast to the Aotus (especially in areas that are surface-exposed) indicated that some monoclonal antibodies against the human CD3 complex will not recognize these Aotus determinants.
- Subjects :
- Amino Acid Sequence
Animals
Base Sequence
CD3 Complex genetics
Humans
Lymphocyte Activation immunology
Molecular Sequence Data
Protein Isoforms genetics
Receptor-CD3 Complex, Antigen, T-Cell genetics
Receptor-CD3 Complex, Antigen, T-Cell immunology
Sequence Alignment
Signal Transduction physiology
Aotidae immunology
CD3 Complex immunology
Protein Isoforms immunology
Subjects
Details
- Language :
- Spanish; Castilian
- ISSN :
- 0120-4157
- Volume :
- 28
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Biomedica : revista del Instituto Nacional de Salud
- Publication Type :
- Academic Journal
- Accession number :
- 18719729