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Using alpha-helical coiled-coils to design nanostructured metalloporphyrin arrays.
- Source :
-
Journal of the American Chemical Society [J Am Chem Soc] 2008 Sep 10; Vol. 130 (36), pp. 11921-7. Date of Electronic Publication: 2008 Aug 19. - Publication Year :
- 2008
-
Abstract
- We have developed a computational design strategy based on the alpha-helical coiled-coil to generate modular peptide motifs capable of assembling into metalloporphyrin arrays of varying lengths. The current study highlights the extension of a two-metalloporphyrin array to a four-metalloporphyrin array through the incorporation of a coiled-coil repeat unit. Molecular dynamics simulations demonstrate that the initial design evolves rapidly to a stable structure with a small rmsd compared to the original model. Biophysical characterization reveals elongated proteins of the desired length, correct cofactor stoichiometry, and cofactor specificity. The successful extension of the two-porphyrin array demonstrates how this methodology serves as a foundation to create linear assemblies of organized electrically and optically responsive cofactors.
- Subjects :
- Amino Acid Sequence
Circular Dichroism
Computer Simulation
Electron Spin Resonance Spectroscopy
Ferric Compounds chemical synthesis
Ferric Compounds chemistry
Metalloporphyrins chemical synthesis
Models, Molecular
Molecular Conformation
Molecular Sequence Data
Molecular Weight
Peptides chemical synthesis
Protein Structure, Secondary
Spectrophotometry, Ultraviolet
Thermodynamics
Metalloporphyrins chemistry
Nanostructures chemistry
Peptides chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1520-5126
- Volume :
- 130
- Issue :
- 36
- Database :
- MEDLINE
- Journal :
- Journal of the American Chemical Society
- Publication Type :
- Academic Journal
- Accession number :
- 18710226
- Full Text :
- https://doi.org/10.1021/ja800697g