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Comparative evaluation of genetic assays to identify oral pre-cancerous fields.
- Source :
-
Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology [J Oral Pathol Med] 2008 Nov; Vol. 37 (10), pp. 599-606. Date of Electronic Publication: 2008 Aug 14. - Publication Year :
- 2008
-
Abstract
- Background: Oral squamous cell carcinomas often develop in a pre-cancerous field, defined as mucosal epithelium with cancer-related genetic alterations, and which may appear as a clinically visible lesion. The test characteristics of three genetic assays that were developed to detect pre-cancerous fields were investigated and compared to histology.<br />Methods: In total, 10 pre-cancerous fields that were not visible at clinical inspection and gave rise to malignant transformation based on an identical TP53 mutation in tumor and mucosal epithelium in the surgical margin, as well as 10 normal oral mucosa specimens were analyzed for numerical chromosomal changes with multiplex ligation-dependent probe amplification (MLPA), for loss of heterozygosity (LOH), with microsatellite PCR and for DNA index alterations with DNA image analysis.<br />Results: No alterations were detected in normal tissue by either of the assays. Both MLPA and LOH assays detected all pre-cancerous fields. DNA cytometry identified aneuploidy in four of 10 pre-cancerous fields, while the corresponding tumors that developed in these fields were shown to be aneuploid.<br />Conclusions: Both the MLPA and LOH assay seem suitable for screening pre-cancerous fields in subjects at high risk for oral cancer even in the absence of clinically abnormal appearing oral mucosa. Measurements of DNA index might be valuable to determine the time to progression.
- Subjects :
- Aneuploidy
DNA Mutational Analysis
Humans
Molecular Diagnostic Techniques
Nucleic Acid Amplification Techniques
Sensitivity and Specificity
Tumor Suppressor Protein p53 genetics
Genetic Testing methods
Leukoplakia, Oral genetics
Loss of Heterozygosity
Mouth Neoplasms genetics
Precancerous Conditions genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1600-0714
- Volume :
- 37
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology
- Publication Type :
- Academic Journal
- Accession number :
- 18705641
- Full Text :
- https://doi.org/10.1111/j.1600-0714.2008.00682.x