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Cytoprotective roles of senescence marker protein 30 against intracellular calcium elevation and oxidative stress.
- Source :
-
Archives of pharmacal research [Arch Pharm Res] 2008 Jul; Vol. 31 (7), pp. 872-7. Date of Electronic Publication: 2008 Aug 14. - Publication Year :
- 2008
-
Abstract
- Senescence marker protein 30 (SMP30) is identified as an important aging marker molecule and known to play multifunctional roles as an intracellular calcium regulatory protein in the signaling process. To elucidate the functional significance of SMP30, we established the stably transfected P19 cell line with SMP30 expression vector. Overexpression of SMP30 slightly suppressed the proliferation of P19 cells. However, SMP30 overexpression was cytoprotective against calcium-mediated stress such as calcium ionophore (A23187), and thapsigargin. We found that SMP30 overexpression reduced the elevated intracellular calcium levels induced by A23187, but not by thapsigargin. In addition, SMP30 transfected P19 cells were more protective to tert-butylhydroperoxide induced cytotoxicity, indicating the antioxidative properties of SMP30. Taken together, our results suggest that external calcium regulation and antioxidant properties are involved in the cytoprotective mechanism of SMP30.
- Subjects :
- Animals
Antioxidants metabolism
Blotting, Western
Calcimycin antagonists & inhibitors
Calcimycin toxicity
Calcium Signaling physiology
Carcinogens antagonists & inhibitors
Carcinogens toxicity
Cell Line, Tumor
Cell Proliferation drug effects
Cell Survival physiology
Mice
Reverse Transcriptase Polymerase Chain Reaction
Thapsigargin antagonists & inhibitors
Thapsigargin toxicity
Transfection
Calcium metabolism
Calcium-Binding Proteins genetics
Calcium-Binding Proteins physiology
Intracellular Signaling Peptides and Proteins genetics
Intracellular Signaling Peptides and Proteins physiology
Oxidative Stress physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0253-6269
- Volume :
- 31
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Archives of pharmacal research
- Publication Type :
- Academic Journal
- Accession number :
- 18704329
- Full Text :
- https://doi.org/10.1007/s12272-001-1240-3