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[Mapping of Rpn4p regions responsible for transcriptional activation of proteasome genes].
- Source :
-
Molekuliarnaia biologiia [Mol Biol (Mosk)] 2008 May-Jun; Vol. 42 (3), pp. 526-32. - Publication Year :
- 2008
-
Abstract
- Rpn4p is positive and negative transcriptional regulator of the ubiquitin-proteasome system. At the same time, it is the extremely short-lived proteasome-associated protein and the proteasome substrate. Proteasome-dependent mechanisms of Rpn4p degradation is studied in details, but mechanisms of its action are not clear, and, first of all, functional domains is not defined. To map Rpn4p functionally important regions, we created a set of its deletion derivatives. Mutant proteins were expressed in rpn4-A yeast strain and their activities were estimated by measuring the mRNA level of proteasomal genes in appropriate transformants. We showed that Rpn4p devoid of its C-terminal region containing the zinc finger DNA-binding domains and its N-terminus, which shares no homology with transactivation domains of known transcription factors, was completely unable to activate transcription. Of two clusters of acid residues, proposed to be activation domains, only second one takes part in transcription. Moreover, deletion of N-terminus, proximal acidic domain or, surprisingly, zinc finger domains leads to Rpn4p stabilization. These data give new insight onto both mechanisms of Rpn4p action and degradation.
- Subjects :
- Amino Acid Sequence genetics
DNA-Binding Proteins genetics
Proteasome Endopeptidase Complex genetics
Proteasome Endopeptidase Complex metabolism
Protein Structure, Tertiary physiology
Repressor Proteins genetics
Saccharomyces cerevisiae genetics
Saccharomyces cerevisiae Proteins genetics
Sequence Deletion
Transcription Factors genetics
Ubiquitin genetics
Ubiquitin metabolism
Zinc Fingers physiology
DNA-Binding Proteins metabolism
Peptide Mapping
Repressor Proteins metabolism
Saccharomyces cerevisiae metabolism
Saccharomyces cerevisiae Proteins metabolism
Transcription Factors metabolism
Subjects
Details
- Language :
- Russian
- ISSN :
- 0026-8984
- Volume :
- 42
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Molekuliarnaia biologiia
- Publication Type :
- Academic Journal
- Accession number :
- 18702312