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The survival of herpes simplex virus in preserved murine corneas.
- Source :
-
Ophthalmic research [Ophthalmic Res] 2008 Oct; Vol. 40 (6), pp. 329-32. Date of Electronic Publication: 2008 Aug 12. - Publication Year :
- 2008
-
Abstract
- Background/aims: This study investigates the survival of HSV in infected mouse corneas, in the conditions of normal human eye bank preservation.<br />Methods: Hundred seventy-two BALB/C mice infected with herpes simplex virus 1 (HSV-1) (KOS) were randomly assigned to either: no preservation in group 1 (n = 62), 31 degrees C preservation for 3 weeks in group 2 (n = 70) or 4 degrees C preservation for 8 days in group 3 (n = 40). The presence of HSV-1 was thereafter detected by viral culture and PCR.<br />Results: In groups 1, 2 and 3, HSV-1 was detected by culture in 22 (35.5%), 1 (1.4%) and 0 (0.0%) of the corneas, and by PCR in 27 (43.7%), 3 (4.2%) and 7 (17.5%) of the corneas respectively. When compared to group 1, HSV was detected significantly less often in groups 2 (p < 0.0001) and 3 (p < 0.0001).<br />Conclusions: HSV-1 DNA undergoes a degradation during corneal preservation.<br /> (Copyright 2008 S. Karger AG, Basel.)
Details
- Language :
- English
- ISSN :
- 1423-0259
- Volume :
- 40
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Ophthalmic research
- Publication Type :
- Academic Journal
- Accession number :
- 18698148
- Full Text :
- https://doi.org/10.1159/000151245