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Stat5 regulates cellular iron uptake of erythroid cells via IRP-2 and TfR-1.
- Source :
-
Blood [Blood] 2008 Nov 01; Vol. 112 (9), pp. 3878-88. Date of Electronic Publication: 2008 Aug 11. - Publication Year :
- 2008
-
Abstract
- Erythropoiesis strictly depends on signal transduction through the erythropoietin receptor (EpoR)-Janus kinase 2 (Jak2)-signal transducer and activator of transcription 5 (Stat5) axis, regulating proliferation, differentiation, and survival. The exact role of the transcription factor Stat5 in erythropoiesis remained puzzling, however, since the first Stat5-deficient mice carried a hypomorphic Stat5 allele, impeding full phenotypical analysis. Using mice completely lacking Stat5--displaying early lethality--we demonstrate that these animals suffer from microcytic anemia due to reduced expression of the antiapoptotic proteins Bcl-x(L) and Mcl-1 followed by enhanced apoptosis. Moreover, transferrin receptor-1 (TfR-1) cell surface levels on erythroid cells were decreased more than 2-fold on erythroid cells of Stat5(-/-) animals. This reduction could be attributed to reduced transcription of TfR-1 mRNA and iron regulatory protein 2 (IRP-2), the major translational regulator of TfR-1 mRNA stability in erythroid cells. Both genes were demonstrated to be direct transcriptional targets of Stat5. This establishes an unexpected mechanistic link between EpoR/Jak/Stat signaling and iron metabolism, processes absolutely essential for erythropoiesis and life.
- Subjects :
- Anemia, Iron-Deficiency genetics
Anemia, Iron-Deficiency metabolism
Anemia, Iron-Deficiency pathology
Animals
Apoptosis
Biological Transport, Active
Embryo Loss
Erythroid Cells pathology
Female
Iron Deficiencies
Liver embryology
Liver metabolism
Liver pathology
Mice
Mice, Inbred C57BL
Mice, Knockout
Myeloid Cell Leukemia Sequence 1 Protein
Pregnancy
Proto-Oncogene Proteins c-bcl-2 metabolism
RNA, Messenger genetics
RNA, Messenger metabolism
STAT5 Transcription Factor deficiency
STAT5 Transcription Factor genetics
Erythroid Cells metabolism
Iron metabolism
Iron Regulatory Protein 2 metabolism
Receptors, Transferrin metabolism
STAT5 Transcription Factor metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1528-0020
- Volume :
- 112
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Blood
- Publication Type :
- Academic Journal
- Accession number :
- 18694996
- Full Text :
- https://doi.org/10.1182/blood-2008-02-138339