Back to Search
Start Over
Using G-deleted vesicular stomatitis virus to probe the innate anti-viral response.
- Source :
-
Journal of virological methods [J Virol Methods] 2008 Nov; Vol. 153 (2), pp. 276-9. Date of Electronic Publication: 2008 Aug 23. - Publication Year :
- 2008
-
Abstract
- A method is described for the use of a G-deleted, conditionally replicating version of vesicular stomatitis virus (VSV) to measure alterations to the innate anti-viral state of cells in vitro. By co-transfecting a gene of interest with an expression vector for VSV-G one can directly measure the replication of the virus in the transfected cells as non-transfected cells will fail to produce infectious progeny due to the lack of glycoprotein in these non-transfected cells. This allows the investigator to focus exclusively on the anti-viral state induced or inhibited in the transfected cells allowing for screening and quantitative analysis of viral or cellular genes that may modify the anti-viral state.
- Subjects :
- Animals
Cell Line
Chlorocebus aethiops
Gene Deletion
Green Fluorescent Proteins genetics
Green Fluorescent Proteins metabolism
Humans
Interferons immunology
Proteins genetics
Proteins immunology
Proteins metabolism
Signal Transduction
Transfection
Vero Cells
Viral Proteins genetics
Viral Proteins immunology
Viral Proteins metabolism
Immunity, Innate
Membrane Glycoproteins genetics
Vesicular stomatitis Indiana virus immunology
Vesicular stomatitis Indiana virus pathogenicity
Viral Envelope Proteins genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0166-0934
- Volume :
- 153
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of virological methods
- Publication Type :
- Academic Journal
- Accession number :
- 18692525
- Full Text :
- https://doi.org/10.1016/j.jviromet.2008.07.001