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L-Carnitine suppresses oleic acid-induced membrane permeability transition of mitochondria.
- Source :
-
Cell biochemistry and function [Cell Biochem Funct] 2008 Oct; Vol. 26 (7), pp. 778-86. - Publication Year :
- 2008
-
Abstract
- Membrane permeability transition (MPT) of mitochondria has an important role in apoptosis of various cells. The classic type of MPT is characterized by increased Ca(2+) transport, membrane depolarization, swelling, and sensitivity to cyclosporin A. In this study, we investigated whether L-carnitine suppresses oleic acid-induced MPT using isolated mitochondria from rat liver. Oleic acid-induced MPT in isolated mitochondria, inhibited endogenous respiration, caused membrane depolarization, and increased large amplitude swelling, and cytochrome c (Cyt. c) release from mitochondria. L-Carnitine was indispensable to beta-oxidation of oleic acid in the mitochondria, and this reaction required ATP and coenzyme A (CoA). In the presence of ATP and CoA, L-carnitine stimulated oleic acid oxidation and suppressed the oleic acid-induced depolarization, swelling, and Cyt. c release. L-Carnitine also contributed to maintaining mitochondrial function, which was decreased by the generation of free fatty acids with the passage of time after isolation. These results suggest that L-carnitine acts to maintain mitochondrial function and suppresses oleic acid-mediated MPT through acceleration of beta-oxidation.<br /> (Copyright (c) 2008 John Wiley & Sons, Ltd.)
- Subjects :
- Adenosine Triphosphate pharmacology
Animals
Cephaloridine pharmacology
Coenzyme A pharmacology
Cytochromes c metabolism
Male
Membrane Potentials drug effects
Mitochondria, Liver metabolism
Mitochondrial Swelling drug effects
Oxidative Phosphorylation drug effects
Oxygen Consumption drug effects
Permeability drug effects
Protective Agents pharmacology
Rats
Rats, Wistar
Time Factors
Carnitine pharmacology
Intracellular Membranes drug effects
Intracellular Membranes metabolism
Mitochondria, Liver drug effects
Oleic Acid pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1099-0844
- Volume :
- 26
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Cell biochemistry and function
- Publication Type :
- Academic Journal
- Accession number :
- 18683897
- Full Text :
- https://doi.org/10.1002/cbf.1506