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The effects of pH on DNA methylation state: In vitro and post-mortem brain studies.
- Source :
-
Journal of neuroscience methods [J Neurosci Methods] 2008 Sep 15; Vol. 174 (1), pp. 123-5. Date of Electronic Publication: 2008 Jul 06. - Publication Year :
- 2008
-
Abstract
- Assessment of methylation state of DNA extracted from brain is becoming one of the most investigated issues in the study of epigenetics and psychopathology. pH effects in brain are known to affect gene transcription, though pH effects on DNA methylation state are unknown. We demonstrate in vitro using an artificially methylated plasmid that DNA methylation state remains stable, even under extreme pH conditions. Next, using two different genomic regions from human DNA, we assess methylation state from both cortical and sub-cortical brain regions using subjects with varying pH levels. No correlation was found between DNA methylation state and pH. These results suggest that DNA methylation state is stable in post-mortem brain.
- Subjects :
- Base Sequence
Brain anatomy & histology
Chromosomal Instability genetics
DNA genetics
DNA metabolism
Humans
Hydrogen-Ion Concentration
Male
Molecular Sequence Data
Plasmids
Postmortem Changes
Brain metabolism
Brain Chemistry genetics
DNA analysis
DNA Methylation
Molecular Biology methods
Neurochemistry methods
Subjects
Details
- Language :
- English
- ISSN :
- 0165-0270
- Volume :
- 174
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of neuroscience methods
- Publication Type :
- Academic Journal
- Accession number :
- 18656499
- Full Text :
- https://doi.org/10.1016/j.jneumeth.2008.06.027